WB (Western Blot)
(Detection of human and mouse GLO1/Glyoxalase I by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GLO1/Glyoxalase I antibody AAA213197 (lot AAA213197-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human, Mouse GLO1/Glyoxalase I Polyclonal Antibody | anti-GLO1 antibody
Rabbit anti-GLO1/Glyoxalase I Antibody, Affinity Purified
Gene Names
GLO1; GLYI; GLOD1; HEL-S-74
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
GLO1/Glyoxalase I, Antibody; Rabbit anti-GLO1/Glyoxalase I Antibody, Affinity Purified; HEL-S-74; S-D-lactoylglutathione methylglyoxal lyase; methylglyoxalase; lactoylglutathione lyase; lactoyl glutathione lyase; Glyoxalase I; glyoxalase domain containing 1; GLYI; glx I; GLOD1; epididymis secretory protein Li 74; aldoketomutase; ketone-aldehyde mutase; Lactoylglutathione lyase; glyoxalase I; anti-GLO1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-GLO1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse GLO1/Glyoxalase I by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GLO1/Glyoxalase I antibody AAA213197 (lot AAA213197-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human and mouse GLO1/Glyoxalase I by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GLO1/Glyoxalase I antibody AAA213197 (lot AAA213197-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human GLO1/Glyoxalase I by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLO1/Glyoxalase Iantibody AAA213197 (lot AAA213197-1) used for IP at 6 ug per reaction. GLO1/Glyoxalase I was also immunoprecipitated by rabbit anti-GLO1/Glyoxalase I antibody For blotting immunoprecipitated GLO1/Glyoxalase I, AAA213197 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human GLO1/Glyoxalase I by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLO1/Glyoxalase Iantibody AAA213197 (lot AAA213197-1) used for IP at 6 ug per reaction. GLO1/Glyoxalase I was also immunoprecipitated by rabbit anti-GLO1/Glyoxalase I antibody For blotting immunoprecipitated GLO1/Glyoxalase I, AAA213197 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Related Product Information for anti-GLO1 antibody
Glyoxylase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione [taken from NCBI Entrez Gene (Gene ID: 2739)].
Product Categories/Family for anti-GLO1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,043 Da
NCBI Official Full Name
lactoylglutathione lyase
NCBI Official Synonym Full Names
glyoxalase I
NCBI Official Symbol
GLO1
NCBI Official Synonym Symbols
GLYI; GLOD1; HEL-S-74
NCBI Protein Information
lactoylglutathione lyase; S-D-lactoylglutathione methylglyoxal lyase; aldoketomutase; epididymis secretory protein Li 74; glx I; glyoxalase domain containing 1; ketone-aldehyde mutase; lactoyl glutathione lyase; methylglyoxalase
UniProt Protein Name
Lactoylglutathione lyase
UniProt Gene Name
GLO1
UniProt Synonym Gene Names
Glx I
UniProt Entry Name
LGUL_HUMAN
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Product Notes
The GLO1 glo1 (Catalog #AAA213197) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-GLO1/Glyoxalase I Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GLO1/Glyoxalase I can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the GLO1 glo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLO1/Glyoxalase I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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