IF (Immunofluorescence)
(AAA323802 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human, Mouse GLU2B Polyclonal Antibody | anti-GLU2B antibody
GLU2B Antibody
Gene Names
PRKCSH; GIIB; PCLD; PLD1; G19P1; PCLD1; PKCSH; AGE-R2; VASAP-60
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GLU2B, Antibody; GLU2B Antibody; 80K-H protein; AGE-binding receptor 2; AGE-R2; G19P1; GLU2B_HUMAN; Glucosidase 2 subunit beta; Glucosidase II beta subunit; Glucosidase II subunit beta; Hepatocystin; PCLD; PKCSH; PLD1; PRKCSH; Protein kinase C substrate 60.1 kDa protein heavy chain; Protein kinase C substrate 80 Kda protein; Protein kinase C substrate 80K-H; Protein kinase C substrate, 80 Kda protein; anti-GLU2B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
GLU2B antibody detects endogenous levels of total GLU2B
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
525
Applicable Applications for anti-GLU2B antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Endoplasmic Reticulum
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Dog
Similarity
Pig (90%), Zebrafish (80%), Bovine (90%), Horse (80%), Sheep (90%), Dog (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA323802 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA323802 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of GLU2B expression in mouse brain lysate)
WB (Western Blot)
(Western blot analysis of GLU2B expression in mouse brain lysate)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain cells, using GLU2B antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain cells, using GLU2B antibody.)
Related Product Information for anti-GLU2B antibody
Function: Regulatory subunit of glucosidase II.
Subunit Structure: Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC (By similarity).
Subunit Structure: Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 59 kD
Predicted: 60 kDa
Predicted: 60 kDa
NCBI Official Full Name
glucosidase 2 subunit beta isoform 2
NCBI Official Synonym Full Names
protein kinase C substrate 80K-H
NCBI Official Symbol
PRKCSH
NCBI Official Synonym Symbols
GIIB; PCLD; PLD1; G19P1; PCLD1; PKCSH; AGE-R2; VASAP-60
NCBI Protein Information
glucosidase 2 subunit beta
UniProt Protein Name
Glucosidase 2 subunit beta
UniProt Gene Name
PRKCSH
UniProt Synonym Gene Names
PKCSH
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Product Notes
The GLU2B prkcsh (Catalog #AAA323802) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GLU2B Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GLU2B can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the GLU2B prkcsh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLU2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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