WB (Western Blot)
(Anti-PACRG Antibody - Western Blot. Western blot of Affinity Purified anti-PACRG antibody shows detection of a band ~40 kD corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 ug of a mouse embryonic fibroblast (MEF) whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1500 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
Rabbit GLUP / PACRG Polyclonal Antibody | anti-GLUP antibody
Rabbit Polyclonal to Human GLUP / PACRG
Gene Names
PACRG; GLUP; PARK2CRG; HAK005771
Reactivity
Chicken, Human, Mouse
Applications
ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
GLUP / PACRG, Antibody; Rabbit Polyclonal to Human GLUP / PACRG; Anti-GLUP / PACRG Antibody (aa204-215) IHC-plus; PACRG; GLUP; HAK005771; PARK2 co-regulated; PARK2CRG; RP3-495O10.2; PARK2 coregulated gene protein; Human GLUP; anti-GLUP antibody
Host
Rabbit
Reactivity
Chicken, Human, Mouse
Clonality
Polyclonal
Specificity
Amino acids 204-215 of Human PACRG protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
296
Applicable Applications for anti-GLUP antibody
ELISA, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
Synthetic peptide from human PACRG.
Immunogen Type
Synthetic peptide
Immunogen
GLUP / PACRG antibody was raised against synthetic peptide from human PACRG.
Antigen Modification
aa204-215
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-PACRG Antibody - Western Blot. Western blot of Affinity Purified anti-PACRG antibody shows detection of a band ~40 kD corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 ug of a mouse embryonic fibroblast (MEF) whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1500 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
WB (Western Blot)
(Anti-PACRG Antibody - Western Blot. Western blot of Affinity Purified anti-PACRG antibody shows detection of a band ~40 kD corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 ug of a mouse embryonic fibroblast (MEF) whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1500 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
IHC (Immunohistochemistry)
(Anti-PACRG antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-PACRG antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,312 Da
NCBI Official Full Name
parkin coregulated gene protein isoform 1
NCBI Official Synonym Full Names
PARK2 co-regulated
NCBI Official Symbol
PACRG
NCBI Official Synonym Symbols
GLUP; PARK2CRG; HAK005771
NCBI Protein Information
parkin coregulated gene protein; parkin co-regulated gene protein; molecular chaperone/chaperonin-binding protein
UniProt Protein Name
Parkin coregulated gene protein
UniProt Gene Name
PACRG
UniProt Synonym Gene Names
GLUP
UniProt Entry Name
PACRG_HUMAN
Similar Products
Product Notes
The GLUP pacrg (Catalog #AAA50763) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human GLUP / PACRG reacts with Chicken, Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GLUP / PACRG can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the GLUP pacrg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLUP / PACRG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.