Glutamic acid decarboxylase Polyclonal Antibody | anti-GAD antibody

Glutamic acid decarboxylase polyclonal antibody

Cat. Num. AAA77007

• Reactivity: Human, Mouse, Rat, Cat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Protein A-affinity chromatography.
• Synonyms: Glutamic acid decarboxylase, Antibody; Glutamic acid decarboxylase polyclonal antibody; GAD; anti-GAD antibody

• Host: Purified from Rabbit serum.
• Reactivity: Human, Mouse, Rat, Cat
• Clonality: Polyclonal
• Specificity: Human, Mouse, Rat, Cat
• Purity/Purification: Protein A-affinity chromatography.
• Form/Format: Liquid. In PBS containing 0.09% sodium azide.
• Sequence Length: 180

• Applicable Applications for anti-GAD antibody: WB (Western Blot), IHC (Immunohistochemistry), ELISA
• Immunogen: Synthetic peptide corresponding to aa 572-585 (D572FLIEEIERLGQDL585) of rat glutamate decarboxylase (GAD65). This sequence is conserved in rat GAD67 and in human GAD-2.
• Preparation and Storage: Ships on Blue Ice.; Store at -20°C.; Avoid freeze/thaw cycles.

IHC (Immunohiostchemistry)

(Immunohistochemistry analysis: Localization of GABAergic neurones within lamina I of cat dorsal horn using rabbit polyclonal antiserum to glutamic acid decarboxylase (GAD65/67).)

WB (Western Blot)

(Western blot analysis of Glutamic Acid Decarboxylase pAb: Lane 1: MW Marker, Lane 2: Rat Brain Tissue Extract, Lane 3: Mouse Brain Tissue Extract.)

Related Product Information for anti-GAD antibody

Background: Glutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA) - the major inhibitory transmitter in higher brain regions. Two molecular forms of GAD (GAD65 and GAD67) are known from rat, cat, pig and man, and both forms are expressed in the CNS and in pancreatic islet endocrine cells. A recent Western blotting study has identified that the isoforms are regionally distributed in the brains of rats and mice. In rat pancreas, GAD65 and GAD67 appear to be differentially localised, GAD65 primarily in insulin-containing ( beta ) cells and GAD67 in glucagon-containing (A) cells. Evidence from knock-out mice suggests that GABA generated by pancreatic GAD isoforms is not critically involved in normal islet cell development and morphology. However, beta -cell-specific GAD expression has been shown to be required for the development of autoimmune diabetes in non-obese diabetic (NOD) mice.

Product Notes: A synthetic peptide with the amino acid sequence: [C]-D-F-L-I-E-E-I-E-R-L-G-Q-D-L was selected from rat glutamate decarboxylase (GAD65; C-terminus residues [Cys]+572-585). This sequence is conserved in rat GAD67 and in human GAD-28. The peptide was conjugated to keyhole limpet hemocyanin via the N-terminal cysteine residue using m-maleimidobenzoic acid N-hydroxysuccinimide. The conjugate was used to immunise New Zealand White rabbits and the resultant antiserum has been extensively characterised by ELISA, Western blot and immunohistochemical techniques.Application dataWestern blottingTest tissues: Mouse and rat brain. Dilution: 1:1000. Protein size: Doublet on SDS-PAGE at approximately 65/67kDa. ImmunohistochemistryTest tissues: Human, cat and rat brain (esp. cerebellum). Fixatives: Several fixative solutions may be used. Aldehyde-combination fixatives (i.e. those containing formaldehyde and glutaraldehyde) usually give satisfactory results. Bouin and Susa fixatives containing 0.1-0.2% glutaraldehyde have been used satisfactorily. Processing: Cryostat and Vibratome®9 sections have been used most commonly. Paraffin wax-embedded tissue has been used successfully. The antiserum has also been used for the ultrastructural detection of GAD using pre-embedding immunocytochemistry. Dilution range: 1:200-1:5000, using overnight incubation and PAP or ABC-peroxidase procedure.

Product Categories/Family for anti-GAD antibody

Cell Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 182942
• NCBI Official Full Name: glutamic acid decarboxylase, partial
• UniProt Protein Name: Glutamate decarboxylase 2
• UniProt Gene Name: GAD2
• UniProt Synonym Gene Names: GAD65; GAD-65
• UniProt Entry Name: DCE2_HUMAN