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IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Rabbit Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Polyclonal Antibody | anti-GAPDH antibody
Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), Antibody; Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH); anti-GAPDH antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GAPDH. It has been selected for its ability to recognize GAPDH in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- TNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRV
MGHHHHHHSGSEF- TNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRV
Applicable Applications for anti-GAPDH antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GAPDH (Thr154~Val324) expressed in E.coli.
Cross Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
IHC (Immunohistchemistry)
(DAB staining on IHC-P; Samples: Human Stomach Tissue)
IHC (Immunohistchemistry)
(DAB staining on IHC-P; Samples: Human Stomach Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
IHC (Immunohistochemistry)
(FITC staining on IHC-P Simple: Hepg2 cells))
IHC (Immunohistochemistry)
(FITC staining on IHC-P Simple: Hepg2 cells))
WB (Western Blot)
(Western Blot: Sample: Recombinant GAPDH, Human.)
WB (Western Blot)
(Western Blot: Sample: Recombinant GAPDH, Human.)
WB (Western Blot)
(Western Blot: Sample: Mouse Placenta lysate; Primary Ab: 1ug/ml Rabbit Anti-Human GAPDH Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Mouse Placenta lysate; Primary Ab: 1ug/ml Rabbit Anti-Human GAPDH Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Liver Tissue; Lane2: Mouse Liver Tissue; Lane3: Mouse Placenta Tissue.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Liver Tissue; Lane2: Mouse Liver Tissue; Lane3: Mouse Placenta Tissue.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,548 Da
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase isoform 2
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
GAPDH
NCBI Official Synonym Symbols
G3PD; GAPD; HEL-S-162eP
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Gene Name
GAPDH
UniProt Synonym Gene Names
GAPD; GAPDH
Similar Products
Product Notes
The GAPDH gapdh (Catalog #AAA20122) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- TNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRV. It is sometimes possible for the material contained within the vial of "Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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