WB (Western Blot)
(Detection of human and mouse GMEB1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GMEB1 antibody AAA212004 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. GMEB1 was also immunoprecipitated by rabbit anti-GMEB1 antibody BL10062, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
Rabbit anti-Human, Mouse GMEB1 Polyclonal Antibody | anti-GMEB1 antibody
Rabbit anti-GMEB1 Antibody, Affinity Purified
Gene Names
GMEB1; PIF96; P96PIF
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
GMEB1, Antibody; Rabbit anti-GMEB1 Antibody, Affinity Purified; P96PIF; PIF p96; parvovirus initiation factor p96; glucocorticoid modulatory element-binding protein 1; DNA-binding protein p96PIF; GMEB-1; PIF96; Glucocorticoid modulatory element-binding protein 1; glucocorticoid modulatory element binding protein 1; anti-GMEB1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-GMEB1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine
Immunogen
Between 523 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse GMEB1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GMEB1 antibody AAA212004 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. GMEB1 was also immunoprecipitated by rabbit anti-GMEB1 antibody BL10062, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
WB (Western Blot)
(Detection of human and mouse GMEB1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GMEB1 antibody AAA212004 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. GMEB1 was also immunoprecipitated by rabbit anti-GMEB1 antibody BL10062, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
IHC (Immunohistochemistry)
(Detection of human and mouse GMEB1 by immunohistochemistry. Sample: FFPE section of human lung carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GMEB1 (Cat. No. AAA212004 Lot1) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human and mouse GMEB1 by immunohistochemistry. Sample: FFPE section of human lung carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-GMEB1 (Cat. No. AAA212004 Lot1) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-GMEB1 antibody
Glucocorticoid modulatory element-binding protein 1 (GMEB1) is a trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. GMEB also binds also to the transferrin receptor promoter. It is an essential auxiliary factor for the replication of parvoviruses [taken from the Universal Protein Resource (UniProt) ww.uniprot.org/uniprot/Q9Y692].
Product Categories/Family for anti-GMEB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,591 Da
NCBI Official Full Name
glucocorticoid modulatory element-binding protein 1 isoform 1
NCBI Official Synonym Full Names
glucocorticoid modulatory element binding protein 1
NCBI Official Symbol
GMEB1
NCBI Official Synonym Symbols
PIF96; P96PIF
NCBI Protein Information
glucocorticoid modulatory element-binding protein 1; GMEB-1; PIF p96; OTTHUMP00000003779; OTTHUMP00000003780; OTTHUMP00000003781; OTTHUMP00000035905; DNA-binding protein p96PIF; parvovirus initiation factor p96
UniProt Protein Name
Glucocorticoid modulatory element-binding protein 1
UniProt Gene Name
GMEB1
UniProt Synonym Gene Names
GMEB-1; PIF p96
UniProt Entry Name
GMEB1_HUMAN
Similar Products
Product Notes
The GMEB1 gmeb1 (Catalog #AAA212004) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-GMEB1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GMEB1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the GMEB1 gmeb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GMEB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.