FCM/FACS (Flow Cytometry)
(GNE Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit GNE Polyclonal Antibody | anti-GNE antibody
GNE Antibody (C-term)
Gene Names
GNE; NM; DMRV; IBM2; Uae1; GLCNE
Reactivity
Human, mouse (Predicted Reactivity: Hamster, Rat)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GNE, Antibody; GNE Antibody (C-term); Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; UDP-N-acetylglucosamine 2-epimerase (hydrolyzing); UDP-GlcNAc-2-epimerase; Uridine diphosphate-N-acetylglucosamine-2-epimerase; N-acetylmannosamine kinase; ManAc kinase; GNE; GLCNE; anti-GNE antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Hamster, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GNE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 580-607 amino acids from the C-terminal region of human GNE.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
580-607
Sequence Length
612
Applicable Applications for anti-GNE antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(GNE Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(GNE Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(GNE Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the GNE antibody detected GNE protein (arrow).)
WB (Western Blot)
(GNE Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the GNE antibody detected GNE protein (arrow).)
Related Product Information for anti-GNE antibody
GNE is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells.
Product Categories/Family for anti-GNE antibody
References
Reinke, S.O., et al. Glycoconj. J. 26(4):415-422(2009)
Tong, Y., et al. PLoS ONE 4 (10), E7165 (2009)
Klootwijk, R.D., et al. FASEB J. 22(11):3846-3852(2008)
Tong, Y., et al. PLoS ONE 4 (10), E7165 (2009)
Klootwijk, R.D., et al. FASEB J. 22(11):3846-3852(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
79275
NCBI Official Full Name
bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform 1
NCBI Official Synonym Full Names
glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
NCBI Official Symbol
GNE
NCBI Official Synonym Symbols
NM; DMRV; IBM2; Uae1; GLCNE
NCBI Protein Information
bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
UniProt Protein Name
Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
UniProt Gene Name
GNE
UniProt Synonym Gene Names
GLCNE
UniProt Entry Name
GLCNE_HUMAN
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Product Notes
The GNE gne (Catalog #AAA284795) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 580-607. The GNE Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Hamster, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's GNE can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GNE gne for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GNE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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