Goat anti Monkey IgG (Fc specific) Secondary Antibody

Goat anti Monkey IgG (Fc specific), conjugated with Horseradish peroxidase

Cat. Num. AAA77592

• Reactivity: Monkey
• Applications: Dot Blot, ELISA, Immunoblot, Western Blot, Immunocytochemistry, Immunohistochemistry
• Synonyms: Goat anti Monkey IgG (Fc specific), Secondary Antibody; Goat anti Monkey IgG (Fc specific), conjugated with Horseradish peroxidase; Goat anti Monkey IgG (Fc specific) secondary antibody

• Host: Goat
• Reactivity: Monkey
• Clonality: Polyclonal
• Specificity: Horseradish peroxidase-conjugated IgG fraction of polyclonal goat antiserum to Monkey IgG,Fc specific.
• Form/Format: Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilizedfrom a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it mayinterfere with the antibody activity.

• Applicable Applications for Goat anti Monkey IgG (Fc specific) secondary antibody: Dot Blot (DB), ELISA (EIA), Immunoblot (IB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)-Paraffin
• Source Note: Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvantis used in the first step of the immunization procedure.
• Conjugation: HRP
• Reactivity Note: Inter-species cross-reactivity is a normal feature of antibodies toimmunoglobulins, since Ig of different species frequently share antigenic determinants.Precipitation reactions have been observed with IgG in Serum of other old-world Monkeys,including Cercopithecus, Cynomolgus and Baboon. The conjugate may also react with IgGof other species as has been observed for Chimpanzee and man.
• Product: Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solutionin phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere withthe antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down toremove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
• Preparation and Storage: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C;prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambienttemperature, spun down again and used to prepare working dilutions by adding sterilephosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day.If a slight precipitation occurs upon storage, this should be removed by centrifugation. It willnot affect the performance of the immunoconjugate.

Related Product Information for Goat anti Monkey IgG (Fc specific) secondary antibody

The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule whichexpresses strict isotypic (class) specificity. It does not react with any non-Ig protein inmonkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Inenzyme-immunocytochemical and immunohistochemical staining for the detection of IgG atthe cellular and subcellular level by staining of appropriately treated cell and tissuesubstrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology andautoimmune diseases; to identify a specific antigen using a reference antibody of monkeyorigin known to be of the IgG isotype in the middle layer of the indirect test procedure; innon-isotopic assay methodology (e.g. ELISA) to measure IgG in monkey serum or otherbody fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibodymust be avoided because it may cause high unspecific background staining and interferewith the specific signal.

Product Categories/Family for Goat anti Monkey IgG (Fc specific) secondary antibody

Conjugated Secondary Antibody

References

1. Sundling C, Zhang Z, Phad GE, Sheng Z, Wang Y, Mascola JR, Li Y, Wyatt RT, Shapiro L,Karlsson Hedestam GB. Single-cell and deep sequencing of IgG-switched macaque B cellsreveal a diverse Ig repertoire following immunization. J Immunol. 2014 Apr 15;192(8):3637-44. doi: 10.4049/jimmunol.1303334. Epub 2014 Mar 12. doi: 10.4049/jimmunol.1303334