IF (Immunofluorescence)
(Immunofluorescence of GOLGIN97 in rat stomach tissue with GOLGIN97 antibody at 20 μg/mL.)
Rabbit GOLGIN97 Polyclonal Antibody | anti-GOLGA1 antibody
GOLGIN97 Antibody
Gene Names
GOLGA1; golgin-97
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column.
Synonyms
GOLGIN97, Antibody; GOLGIN97 Antibody; Golgin subfamily A member 1, GOLGA1, GOLGIN A1, GOLGIN-97; anti-GOLGA1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
GOLGIN97 antibody is human, mouse and rat reactive. At least two isoforms of GOLGIN97 are known to exist; this antibody will detect only the longer isoform. GOLGIN97 antibody is predicted to not cross-react with other GOLGIN family proteins.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
767
Applicable Applications for anti-GOLGA1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
GOLGIN97 antibody was raised against a 17 amino acid peptide near the amino terminus of human GOLGIN97.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
Human Stomach Tissue Lysate
Conjugate
Unconjugated
MW Explanation
Heavily post-translationally modifed, multiple independent GOLGIN97 antibodies show bands at ~120kDa
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
IF (Immunofluorescence)
(Immunofluorescence of GOLGIN97 in rat stomach tissue with GOLGIN97 antibody at 20 μg/mL.)
IF (Immunofluorescence)
(Immunofluorescence of GOLGIN97 in rat stomach tissue with GOLGIN97 antibody at 20 μg/mL.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of GOLGIN97 in rat stomach tissue with GOLGIN97 antibody at 5 μg/mL.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of GOLGIN97 in rat stomach tissue with GOLGIN97 antibody at 5 μg/mL.)
WB (Western Blot)
(Western blot analysis of GOLGIN97 in human stomach tissue lysate with GOLGIN97 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking petpide.)
WB (Western Blot)
(Western blot analysis of GOLGIN97 in human stomach tissue lysate with GOLGIN97 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking petpide.)
Related Product Information for anti-GOLGA1 antibody
GOLGIN97 was initially isolated as a Golgi complex autoantigen associated with Sjogren's syndrome, a chronic autoimmune disease in which the body's white blood cells destroy the exocrine glands, specifically the salivary and lacrimal glands (1,2). GOLGIN97 plays a role as a tethering molecule in endosome to the trans-Golgi network (TGN) retrograde traffic (3). FIP1/RCP binding to GOLGIN97 is required for tethering and fusion of recycling endosome retrograde transport vesicles to the TGN (4).
Product Categories/Family for anti-GOLGA1 antibody
References
Griffith KJ, Chan EK, Lung CC, et al. Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjogren's syndrome. Arthritis Rheum. 1997; 40:1693-702. Borchers AT, Naguwa SM, Keen CL, et al. Immunopathogenesis of Sjogren's syndrome. Clin. Rev. Allergy Immunol. 2003; 25:89-104. Lu L, Tai G, and Hong W. Autoantigen Golgin-97, and effector of ARL1 GTPase, participates in traffic from the endosome to the trans-Golgi network. Mol. Biol. Cell 2004; 15:4426-43. Jing J, Junutula JR, Wu C, et al. FIP1/RCP binding to Golgin-97 regulates retrograde transport from recycling endosomes to the trans-Golgi network. Mol. Biol. Cell 2010; 21:3041-53.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 84 kDa
Observed: 120 kDa
Observed: 120 kDa
NCBI Official Full Name
golgin subfamily A member 1
NCBI Official Synonym Full Names
golgin A1
NCBI Official Symbol
GOLGA1
NCBI Official Synonym Symbols
golgin-97
NCBI Protein Information
golgin subfamily A member 1
UniProt Protein Name
Golgin subfamily A member 1
UniProt Gene Name
GOLGA1
UniProt Entry Name
GOGA1_HUMAN
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Product Notes
The GOLGA1 golga1 (Catalog #AAA40824) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GOLGIN97 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GOLGIN97 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the GOLGA1 golga1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GOLGIN97, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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