IHC (Immunohiostchemistry)
(AAA328937 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit GOLPH4 Polyclonal Antibody | anti-GOLIM4 antibody
GOLPH4 Antibody
Gene Names
GOLIM4; P138; GIMPC; GOLPH4; GPP130
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
GOLPH4, Antibody; GOLPH4 Antibody; 130 kDa golgi localized phosphoprotein; cis; cis Golgi localized calcium binding protein; GIMPc; Golgi integral membrane protein 4; Golgi integral membrane protein; Golgi phosphoprotein 4; Golgi phosphoprotein of 130 kDa; Golgi-localized phosphoprotein of 130 kDa; GOLI4_HUMAN; golim4; GOLPH 4; GPP 130; GPP130; P 138; P138; type II Golgi membrane protein; anti-GOLIM4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
GOLPH4 Antibody detects endogenous levels of total GOLPH4.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
696
Applicable Applications for anti-GOLIM4 antibody
ELISA
Immunogen
A synthesized peptide derived from human GOLPH4, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA328937 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA328937 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from EC304 cells, using GOLPH4 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from EC304 cells, using GOLPH4 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-GOLIM4 antibody
Function: Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi.
Post Translational Modifications: Phosphorylated probably by c-AMP-dependent kinases in its lumenal part.O-glycosylated; modified by sialic acid residues.N-glycosylated; N-glycans are probably of the complex type and modified by sialic acid residues.
Subcellular Location: Golgi apparatus>Golgi stack membrane>Single-pass type II membrane protein. Endosome membrane>Single-pass type II membrane protein. Membrane>Lipid-anchor. Note: Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome.
Similarity: Belongs to the GOLIM4 family.
Post Translational Modifications: Phosphorylated probably by c-AMP-dependent kinases in its lumenal part.O-glycosylated; modified by sialic acid residues.N-glycosylated; N-glycans are probably of the complex type and modified by sialic acid residues.
Subcellular Location: Golgi apparatus>Golgi stack membrane>Single-pass type II membrane protein. Endosome membrane>Single-pass type II membrane protein. Membrane>Lipid-anchor. Note: Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome.
Similarity: Belongs to the GOLIM4 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)81kD.
Predicted Molecular Weight: (Calculated)82kDa.
Predicted Molecular Weight: (Calculated)82kDa.
NCBI Official Full Name
Golgi integral membrane protein 4 isoform 1
NCBI Official Synonym Full Names
golgi integral membrane protein 4
NCBI Official Symbol
GOLIM4
NCBI Official Synonym Symbols
P138; GIMPC; GOLPH4; GPP130
NCBI Protein Information
Golgi integral membrane protein 4
UniProt Protein Name
Golgi integral membrane protein 4
UniProt Gene Name
GOLIM4
UniProt Synonym Gene Names
GIMPC; GOLPH4; GPP130; GIMPc; Golgi phosphoprotein of 130 kDa
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Product Notes
The GOLIM4 golim4 (Catalog #AAA328937) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GOLPH4 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's GOLPH4 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the GOLIM4 golim4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GOLPH4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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