IF (Immunofluorescence)
(AAA329080 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329080) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit GP210 Polyclonal Antibody | anti-NUP210 antibody
GP210 Antibody
Gene Names
NUP210; GP210; POM210
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(88%), Horse(88%), Rabbit(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
GP210, Antibody; GP210 Antibody; FLJ22389; GP 210; KIAA0906; Nuclear envelope pore membrane protein POM 210; Nuclear pore membrane glycoprotein 210; Nuclear pore protein gp210; Nucleoporin 210; Nucleoporin 210kDa; Nucleoporin Nup210; Nucleoporin210; NUP 210; Nup210; PO210_HUMAN; POM 210; POM210; Pore membrane protein of 210 kDa; anti-NUP210 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Horse(88%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(88%), Horse(88%), Rabbit(100%), Dog(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
GP210 Antibody detects endogenous levels of total GP210.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
1887
Applicable Applications for anti-NUP210 antibody
ELISA
Immunogen
A synthesized peptide derived from human GP210, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA329080 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329080) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA329080 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329080) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
WB (Western Blot)
(Western blot analysis of extracts from Rat liver, using GP210 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Rat liver, using GP210 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-NUP210 antibody
Function: Nucleoporin essential for nuclear pore assembly and fusion, nuclear pore spacing, as well as structural integrity.
Post Translational Modifications: N-glycosylated, but not all potential glycosylation sites may be used. Contains high-mannose type oligosaccharides (By similarity).Phosphorylated at Ser-1881 in mitosis specifically; not phosphorylated in interphase.
Subcellular Location: Nucleus>Nuclear pore complex. Nucleus membrane>Single-pass type I membrane protein. Endoplasmic reticulum membrane>Single-pass type I membrane protein.
Tissue Specificity: Ubiquitous expression, with highest levels in lung, liver, pancreas, testis, and ovary, intermediate levels in brain, kidney, and spleen, and lowest levels in heart and skeletal muscle.
Subunit Structure: Forms dimers and possibly higher-order oligomers.
Similarity: Belongs to the NUP210 family.
Post Translational Modifications: N-glycosylated, but not all potential glycosylation sites may be used. Contains high-mannose type oligosaccharides (By similarity).Phosphorylated at Ser-1881 in mitosis specifically; not phosphorylated in interphase.
Subcellular Location: Nucleus>Nuclear pore complex. Nucleus membrane>Single-pass type I membrane protein. Endoplasmic reticulum membrane>Single-pass type I membrane protein.
Tissue Specificity: Ubiquitous expression, with highest levels in lung, liver, pancreas, testis, and ovary, intermediate levels in brain, kidney, and spleen, and lowest levels in heart and skeletal muscle.
Subunit Structure: Forms dimers and possibly higher-order oligomers.
Similarity: Belongs to the NUP210 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)205kD.
Predicted Molecular Weight: (Calculated)205kDa.
Predicted Molecular Weight: (Calculated)205kDa.
NCBI Official Full Name
nuclear pore membrane glycoprotein 210
NCBI Official Synonym Full Names
nucleoporin 210
NCBI Official Symbol
NUP210
NCBI Official Synonym Symbols
GP210; POM210
NCBI Protein Information
nuclear pore membrane glycoprotein 210
UniProt Protein Name
Nuclear pore membrane glycoprotein 210
UniProt Gene Name
NUP210
UniProt Synonym Gene Names
KIAA0906; Nuclear pore protein gp210; POM210
UniProt Entry Name
PO210_HUMAN
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Product Notes
The NUP210 nup210 (Catalog #AAA329080) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GP210 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(88%), Horse(88%), Rabbit(100%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's GP210 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the NUP210 nup210 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GP210, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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