FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of CEM cells using Anti-GPM6B Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.)
Rabbit anti-Human GPM6B Polyclonal Antibody | anti-GPM6B antibody
Anti-GPM6B Antibody
Gene Names
GPM6B; M6B
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
GPM6B, Antibody; Anti-GPM6B Antibody; anti-GPM6B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of GPM6B protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH7.3, 30% glycerol, and 0.01% sodium azide.
Applicable Applications for anti-GPM6B antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human GPM6B. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of CEM cells using Anti-GPM6B Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of CEM cells using Anti-GPM6B Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.)
WB (Western Blot)
(Western blot analysis of GPM6B expression in ZR751 (A), CEM (B) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 35 kD))
WB (Western Blot)
(Western blot analysis of GPM6B expression in ZR751 (A), CEM (B) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 35 kD))
Related Product Information for anti-GPM6B antibody
Rabbit polyclonal antibody to GPM6B
Product Categories/Family for anti-GPM6B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
28,989 Da
NCBI Official Full Name
Glycoprotein M6B
NCBI Official Synonym Full Names
glycoprotein M6B
NCBI Official Symbol
GPM6B
NCBI Official Synonym Symbols
M6B
NCBI Protein Information
neuronal membrane glycoprotein M6-b; protolipid M6B
UniProt Protein Name
Neuronal membrane glycoprotein M6-b
UniProt Gene Name
GPM6B
UniProt Synonym Gene Names
M6B; M6b
UniProt Entry Name
GPM6B_HUMAN
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Product Notes
The GPM6B gpm6b (Catalog #AAA265406) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GPM6B Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPM6B can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the GPM6B gpm6b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPM6B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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