Rabbit anti-Human GPNMB Polyclonal Antibody | anti-GPNMB antibody

GPNMB Antibody (C-term)

Cat. Num. AAA288949

• Gene Names: GPNMB; NMB; HGFIN
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: GPNMB, Antibody; GPNMB Antibody (C-term); Transmembrane glycoprotein NMB; Transmembrane glycoprotein HGFIN; GPNMB; HGFIN; NMB; anti-GPNMB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This GPNMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-569 amino acids from the C-terminal region of human GPNMB.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 560

• Applicable Applications for anti-GPNMB antibody: ELISA, WB (Western Blot)
• Antigen Region: 541-569
• Function: Could be a melanogenic enzyme.
• Cellular Location: Cell membrane; Single-pass type I membrane protein Melanosome membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
• Tissue Location: Widely expressed, but very low expression, if any, in the brain (PubMed:12609765, PubMed:16609006). Expressed in the epidermis with higher levels in melanocytes compared with keratinocytes and Langerhans cells (at protein level) (PubMed:29336782). Expressed in peripheral blood, but not bone marrow mononuclear cells (PubMed:12609765). Expressed in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level) (PubMed:16489096). May be overexpressed in various cancers, including melanoma and glioblastoma multiforme (PubMed:7814155, PubMed:16489096, PubMed:16609006).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Anti-GPNMB Antibody (C-term) at 1:500 dilution + PC-3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 64 kDa Blocking/Dilution buffer: 5% NFDM/TBST)

Related Product Information for anti-GPNMB antibody

The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-GPNMB antibody

Cancer; Immunology

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Williams, M.D., et al. Melanoma Res. 20(3):184-190(2010)
Hoashi, T., et al. FASEB J. 24(5):1616-1629(2010)
Rose, A.A., et al. Clin. Cancer Res. 16(7):2147-2156(2010)
Rose, A.A., et al. PLoS ONE 5 (8), E12093 (2010) :

NCBI and Uniprot Product Information

• NCBI GI #: 52694752
• NCBI GeneID: 10457
• NCBI Accession #: NP_001005340.1; NP_002501.1
• NCBI GenBank Nucleotide #: NM_001005340.1
• UniProt Accession #: Q14956; Q6UVX1; Q8N1A1; A4D155
• Molecular Weight: 63923
• NCBI Official Full Name: transmembrane glycoprotein NMB isoform a
• NCBI Official Synonym Full Names: glycoprotein (transmembrane) nmb
• NCBI Official Symbol: GPNMB
• NCBI Official Synonym Symbols: NMB; HGFIN
• NCBI Protein Information: transmembrane glycoprotein NMB
• UniProt Protein Name: Transmembrane glycoprotein NMB
• UniProt Gene Name: GPNMB
• UniProt Synonym Gene Names: HGFIN; NMB
• UniProt Entry Name: GPNMB_HUMAN