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WB (Western Blot)
(Figure 1. Western blot analysis of Gpnmb using anti-Gpnmb antibody.)
Rabbit GPNMB/Gpnmb Polyclonal Antibody | anti-Gpnmb antibody
Anti-GPNMB/Gpnmb Antibody
Gene Names
Gpnmb; ipd; Dchil; DC-HIL
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
GPNMB/Gpnmb, Antibody; Anti-GPNMB/Gpnmb Antibody; Transmembrane glycoprotein NMB; DC-HIL; Dendritic cell-associated transmembrane protein; Osteoactivin; Gpnmb; Dchil; Hgfin;;Nmb; anti-Gpnmb antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
574
Applicable Applications for anti-Gpnmb antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml (Mouse, Rat)
IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human, Mouse)
Direct ELISA: 0.1-0.5ug/ml (Mouse)
Tested Species: In-house tested species with positive results.
IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human, Mouse)
Direct ELISA: 0.1-0.5ug/ml (Mouse)
Tested Species: In-house tested species with positive results.
Immunogen
E Coli-derived mouse GPNMB/Gpnmb recombinant protein (Position: R164-D564).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Figure 1. Western blot analysis of Gpnmb using anti-Gpnmb antibody.)
WB (Western Blot)
(Figure 1. Western blot analysis of Gpnmb using anti-Gpnmb antibody.)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IF (Immunofluorescence)
IF (Immunofluorescence)
FCM (Flow Cytometry)
FCM (Flow Cytometry)
FCM (Flow Cytometry)
FCM (Flow Cytometry)
Related Product Information for anti-Gpnmb antibody
Description: Rabbit IgG polyclonal antibody for GPNMB/Gpnmb detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human; Mouse; Rat.
Background: Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. It is mapped to 6 B2.3; 6 23.82 cM. The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
Background: Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. It is mapped to 6 B2.3; 6 23.82 cM. The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
References
1. Lu, H., Wang, X., Pullen, M., Guan, H., Chen, H., Sahu, S., Zhang, B., Chen, H., Williams, R. W., Geisert, E. E., Lu, L., Jablonski, M. M. Genetic dissection of the Gpnmb network in the eye. Invest. Ophthal. Vis. Sci. 52: 4132-4142, 2011.
2. Weterman, M. A. J., Ajubi, N., van Dinter, I. M. R., Degen, W. G. J., van Muijen, G. N. P., Ruiter, D. J., Bloemers, H. P. J. Nmb, a novel gene, is expressed in low-metastatic human melanoma cell lines and xenografts. Int. J. Cancer 60: 73-81, 1995.
3. Yang, C.-F., Lin, S.-P., Chiang, C.-P., Wu, Y.-H., H'ng, W. S., Chang, C.-P., Chen, Y.-T., Wu, J.-Y. Loss of GPNMB causes autosomal-recessive amyloidosis cutis dyschromica in humans. Am. J. Hum. Genet. 102: 219-232, 2018.
2. Weterman, M. A. J., Ajubi, N., van Dinter, I. M. R., Degen, W. G. J., van Muijen, G. N. P., Ruiter, D. J., Bloemers, H. P. J. Nmb, a novel gene, is expressed in low-metastatic human melanoma cell lines and xenografts. Int. J. Cancer 60: 73-81, 1995.
3. Yang, C.-F., Lin, S.-P., Chiang, C.-P., Wu, Y.-H., H'ng, W. S., Chang, C.-P., Chen, Y.-T., Wu, J.-Y. Loss of GPNMB causes autosomal-recessive amyloidosis cutis dyschromica in humans. Am. J. Hum. Genet. 102: 219-232, 2018.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Transmembrane glycoprotein NMB
NCBI Official Synonym Full Names
glycoprotein (transmembrane) nmb
NCBI Official Symbol
Gpnmb
NCBI Official Synonym Symbols
ipd; Dchil; DC-HIL
NCBI Protein Information
transmembrane glycoprotein NMB
UniProt Protein Name
Transmembrane glycoprotein NMB
UniProt Gene Name
Gpnmb
UniProt Synonym Gene Names
Dchil; Hgfin; Nmb
UniProt Entry Name
GPNMB_MOUSE
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Product Notes
The Gpnmb gpnmb (Catalog #AAA19201) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GPNMB/Gpnmb Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GPNMB/Gpnmb can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA). WB: 0.25-0.5ug/ml (Mouse, Rat) IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse) ICC: 2ug/ml (Human) IF: 2ug/ml (Human) FC/FACS: 1-3ug/1x106 cells (Human, Mouse) Direct ELISA: 0.1-0.5ug/ml (Mouse) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the Gpnmb gpnmb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPNMB/Gpnmb, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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