IF (Immunofluorescence)
(AAA325504 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human GPR100 Polyclonal Antibody | anti-GPR100 antibody
GPR100 Antibody
Gene Names
RXFP4; GPR100; RLN3R2; RXFPR4; GPCR142
Reactivity
Human
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GPR100, Antibody; GPR100 Antibody; G protein coupled receptor 100; G-protein coupled receptor 100; G-protein coupled receptor GPCR142; GPCR142; Insulin-like peptide INSL5 receptor; Relaxin 3 receptor 2; Relaxin family peptide receptor 4; Relaxin-3 receptor 2; Relaxin/insulin like family peptide receptor 4; RL3R2_HUMAN; RLN3 receptor 2; RLN3R2; Rxfp4; RXFPR4; anti-GPR100 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
GPR100 antibody detects endogenous levels of total GPR100
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
374
Applicable Applications for anti-GPR100 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane
Tissue Specificity
Expressed in a broader range of tissues including brain, kidney, testis, thymus, placenta, prostate, salivary gland, thyroid and colon.
Predicted Cross Reactivity
Sheep
Similarity
Sheep (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA325504 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA325504 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from A549 cells, using GPR100 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from A549 cells, using GPR100 antibody.)
Related Product Information for anti-GPR100 antibody
Function: High affinity receptor for INSL5. Also acts as receptor for RLN3/relaxin-3, as well as bradykinin and kallidin. Binding of the ligand inhibit cAMP accumulation.
Similarity: Belongs to the G-protein coupled receptor 1 family.
Similarity: Belongs to the G-protein coupled receptor 1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 38 kD
Predicted: 42 kDa
Predicted: 42 kDa
NCBI Official Full Name
relaxin-3 receptor 2
NCBI Official Synonym Full Names
relaxin family peptide/INSL5 receptor 4
NCBI Official Symbol
RXFP4
NCBI Official Synonym Symbols
GPR100; RLN3R2; RXFPR4; GPCR142
NCBI Protein Information
relaxin-3 receptor 2
UniProt Protein Name
Relaxin-3 receptor 2
UniProt Gene Name
RXFP4
UniProt Synonym Gene Names
GPR100; RLN3R2; RLN3 receptor 2
Similar Products
Product Notes
The GPR100 rxfp4 (Catalog #AAA325504) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR100 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPR100 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the GPR100 rxfp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR100, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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