IHC (Immunohiostchemistry)
(AAA323570 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human GPR44 Polyclonal Antibody | anti-GPR44 antibody
GPR44 Antibody
Gene Names
PTGDR2; DP2; DL1R; CD294; CRTH2; GPR44
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GPR44, Antibody; GPR44 Antibody; CD294; CD294 antigen; Chemoattractant receptor homologous molecule expressed on Th2 cells; chemoattractant receptor-homologous; Chemoattractant receptor-homologous molecule expressed on Th2 cells; CRTH 2; CRTH2; DL1R; DP2 prostaglandin receptor; G Protein Coupled Receptor 44; GPR 44; Gpr44; GPR44_HUMAN; PTGDR2; Putative G protein coupled receptor 44; Putative G-protein coupled receptor 44; anti-GPR44 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
GPR44 antibody detects endogenous levels of total GPR44
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
395
Applicable Applications for anti-GPR44 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane
Tissue Specificity
Widespread expression. High expression in stomach, small intestine, heart and thymus. Intermediate expression in colon, spinal cord and peripheral blood and low expression in brain, skeletal muscle and spleen. Expressed also on Th2-and Tc2-type cells, eosinophils and basophils.
Conjugation
Unconjugated
IHC (Immunohiostchemistry)
(AAA323570 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA323570 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of GPR44 expression in Jurkat cell lysate, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of GPR44 expression in Jurkat cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-GPR44 antibody
Description: Orphan receptor.
Function: Receptor for prostaglandin D2 (PGD2). Coupled to the G(i)-protein. Receptor activation may result in pertussis toxin-sensitive decreases in cAMP levels and Ca2+ mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induced receptor internalization. CRTH2 internalization can be regulated by diverse kinases such as, PKC, PKA, GRK2, GPRK5/GRK5 and GRK6. Receptor activation is responsible, at least in part, in immune regulation and allergic/inflammation responses.
Post-translational Modifications: Phosphorylated.
Similarity: The 330-DSEL-333 motif is involved in the recycling of PTGDR2 to the cell surface after agonist-induced internalization. This motif seems to be required for GRK2 and GPRK5/GRK5 to promote agonist-induced internalization. Thr-347 is a major site for PKC-induced internalization of the receptor. Belongs to the G-protein coupled receptor 1 family.
Function: Receptor for prostaglandin D2 (PGD2). Coupled to the G(i)-protein. Receptor activation may result in pertussis toxin-sensitive decreases in cAMP levels and Ca2+ mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induced receptor internalization. CRTH2 internalization can be regulated by diverse kinases such as, PKC, PKA, GRK2, GPRK5/GRK5 and GRK6. Receptor activation is responsible, at least in part, in immune regulation and allergic/inflammation responses.
Post-translational Modifications: Phosphorylated.
Similarity: The 330-DSEL-333 motif is involved in the recycling of PTGDR2 to the cell surface after agonist-induced internalization. This motif seems to be required for GRK2 and GPRK5/GRK5 to promote agonist-induced internalization. Thr-347 is a major site for PKC-induced internalization of the receptor. Belongs to the G-protein coupled receptor 1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 66 kDa
Predicted: 44 kDa
Predicted: 44 kDa
NCBI Official Full Name
prostaglandin D2 receptor 2
NCBI Official Synonym Full Names
prostaglandin D2 receptor 2
NCBI Official Symbol
PTGDR2
NCBI Official Synonym Symbols
DP2; DL1R; CD294; CRTH2; GPR44
NCBI Protein Information
prostaglandin D2 receptor 2
UniProt Protein Name
Prostaglandin D2 receptor 2
UniProt Gene Name
PTGDR2
UniProt Synonym Gene Names
CRTH2; DL1R; GPR44
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Product Notes
The GPR44 ptgdr2 (Catalog #AAA323570) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR44 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPR44 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GPR44 ptgdr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR44, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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