IF (Immunofluorescence)
(AAA323575 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA323575) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
Rabbit anti-Human, Mouse GPR62 Polyclonal Antibody | anti-GPR62 antibody
GPR62 Antibody
Gene Names
GPR62; GPCR8; KPG_005
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
peptide affinity purification
Synonyms
GPR62, Antibody; GPR62 Antibody; G protein coupled receptor GPCR8; G protein coupled receptor KPG_005; G protein-coupled receptor 62; G-protein coupled receptor GPCR8; G-protein coupled receptor KPG_005; GPCR8; GPR62; GPR62_HUMAN; hGPCR8; KPG_005; MGC26943; Probable G protein coupled receptor 62; Probable G-protein coupled receptor 62; anti-GPR62 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
GPR62 antibody detects endogenous levels of total GPR62
Purity/Purification
peptide affinity purification
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
368
Applicable Applications for anti-GPR62 antibody
ELISA, WB (Western Blot)
Immunogen
A synthesized peptide derived from human GPR62, corresponding to a region with C-terminal amino acids.
Important
For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IF (Immunofluorescence)
(AAA323575 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA323575) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
IF (Immunofluorescence)
(AAA323575 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA323575) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of GPR62 expression in HepG2 whole cell lysate.The lane on the left was treated with the antigen specific peptide.)
WB (Western Blot)
(Western blot analysis of GPR62 expression in HepG2 whole cell lysate.The lane on the left was treated with the antigen specific peptide.)
Related Product Information for anti-GPR62 antibody
Function: Orphan G-protein coupled receptor. Constitutively activates the G(q/11)/inositol phosphate and the G(s)-alpha/cAMP signaling pathways (PubMed:28827538). Has spontaneous activity for beta-arrestin recruitment (PubMed:28827538). Shows a reciprocal modulation of signaling functions with the melatonin receptor MTNR1B most likely through receptor heteromerization (PubMed:28827538).
Subunit Structure: Homodimers (By similarity). Forms heterodimer with MTNR1B (PubMed:28827538). Interacts with ARRB1 and ARRB2 in a spontaneous and agonist-independent manner; leading to the internalization of GPR62 in the endosomal compartment (PubMed:28827538).
Similarity: Lacks the conserved DRY and BBXXB motifs. The restoration of these motifs affects its constitutive activity. Belongs to the G-protein coupled receptor 1 family.
Subunit Structure: Homodimers (By similarity). Forms heterodimer with MTNR1B (PubMed:28827538). Interacts with ARRB1 and ARRB2 in a spontaneous and agonist-independent manner; leading to the internalization of GPR62 in the endosomal compartment (PubMed:28827538).
Similarity: Lacks the conserved DRY and BBXXB motifs. The restoration of these motifs affects its constitutive activity. Belongs to the G-protein coupled receptor 1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38 kDa
NCBI Official Full Name
G-protein coupled receptor 62
NCBI Official Synonym Full Names
G protein-coupled receptor 62
NCBI Official Symbol
GPR62
NCBI Official Synonym Symbols
GPCR8; KPG_005
NCBI Protein Information
G-protein coupled receptor 62
UniProt Protein Name
G-protein coupled receptor 62
UniProt Gene Name
GPR62
UniProt Synonym Gene Names
hGPCR8
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Product Notes
The GPR62 gpr62 (Catalog #AAA323575) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR62 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GPR62 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GPR62 gpr62 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR62, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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