WB (Western Blot)
(GPR97 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the GPR97 antibody detected the GPR97 protein (arrow).)
Rabbit anti-Human GPR97 Polyclonal Antibody | anti-GPR97 antibody
GPR97 Antibody (N-term)
Gene Names
ADGRG3; PB99; GPR97; PGR26
Reactivity
Human
Applications
ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
GPR97, Antibody; GPR97 Antibody (N-term); Probable G-protein coupled receptor 97; G-protein coupled receptor PGR26; GPR97; PGR26; anti-GPR97 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This GPR97 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 amino acids from the N-terminal region of human GPR97.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
103-130
Sequence Length
549
Applicable Applications for anti-GPR97 antibody
ELISA, WB (Western Blot)
Function
Adhesion G-protein coupled receptor (aGPCR) for glucocorticoid hormones such as cortisol, cortisone and 11- deoxycortisol (PubMed:33408414). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed:33408414). ADGRG3/GPR97 is coupled to G(o)/GNAO1 G proteins and mediates signaling by inhibiting adenylate cyclase activity (PubMed:33408414). May also signal through G-alpha(q)-proteins; additional evidence are however required to confirm this result in vivo (PubMed:22575658). Plays a role in the regulation of various processes including B-cell development, inflammation or innate immunity (PubMed:30559745, PubMed:36302784). Regulates migration of lymphatic endothelial cells in vitro via the small GTPases RhoA and CDC42 (PubMed:24178298). Antibody ligation leads to the production and activation of antimicrobial mediators like reactive oxygen species (ROS) and myeloperoxidase (MPO) as well as enhanced bacteria uptake and killing by granulocytes (PubMed:30559745). Additionally, collaborates with protease-activated receptor 2/PAR2 to stimulate neutrophil-driven antimicrobial responses and endothelial cell activation (PubMed:36302784).
Cellular Location
Cell membrane; Multi-pass membrane protein
Tissue Location
Expressed in cultured primary dermal lymphatic endothelial cells (PubMed:24178298). Highly expressed in polymorphonuclear cells (PMNs) including neutrophilic, eosinophilic, and basophilic granulocytes (PubMed:30559745)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(GPR97 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the GPR97 antibody detected the GPR97 protein (arrow).)
WB (Western Blot)
(GPR97 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the GPR97 antibody detected the GPR97 protein (arrow).)
Related Product Information for anti-GPR97 antibody
GPR97 is an orphan receptor.
Product Categories/Family for anti-GPR97 antibody
References
Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010) :
Bjarnadottir, T.K., et al. Genomics 84(1):23-33(2004)
Vassilatis, D.K., et al. Proc. Natl. Acad. Sci. U.S.A. 100(8):4903-4908(2003)
Fredriksson, R., et al. FEBS Lett. 531(3):407-414(2002)
Kuznicki, J., et al. Cell Biol. Int. Rep. 3(1):17-23(1979)
Bjarnadottir, T.K., et al. Genomics 84(1):23-33(2004)
Vassilatis, D.K., et al. Proc. Natl. Acad. Sci. U.S.A. 100(8):4903-4908(2003)
Fredriksson, R., et al. FEBS Lett. 531(3):407-414(2002)
Kuznicki, J., et al. Cell Biol. Int. Rep. 3(1):17-23(1979)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60861
NCBI Official Full Name
probable G-protein coupled receptor 97 isoform 1
NCBI Official Synonym Full Names
adhesion G protein-coupled receptor G3
NCBI Official Symbol
ADGRG3
NCBI Official Synonym Symbols
PB99; GPR97; PGR26
NCBI Protein Information
probable G-protein coupled receptor 97
UniProt Protein Name
Probable G-protein coupled receptor 97
UniProt Gene Name
GPR97
UniProt Synonym Gene Names
PGR26
UniProt Entry Name
GPR97_HUMAN
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Product Notes
The GPR97 gpr97 (Catalog #AAA287475) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 103-130. The GPR97 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPR97 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GPR97 gpr97 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR97, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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