WB (Western Blot)
(Detection of human GPRC5A by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GPRC5A antibody AAA212694 (lot AAA212694-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human GPRC5A Polyclonal Antibody | anti-GPRC5A antibody
Rabbit anti-GPRC5A Antibody, Affinity Purified
Gene Names
GPRC5A; RAI3; RAIG1; GPCR5A
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
GPRC5A, Antibody; Rabbit anti-GPRC5A Antibody, Affinity Purified; RAIG-1; orphan G-protein-coupling receptor PEIG-1; TPA induced gene 1; TIG1; retinoic acid-induced protein 3; retinoic acid-induced gene 1 protein; retinoic acid responsive; retinoic acid induced 3; RAI3; phorbol ester induced protein-1; GPCR5A; PEIG-1; RAIG1; G-protein coupled receptor family C group 5 member A; phorbol ester induced gene 1; Retinoic acid-induced protein 3; G protein-coupled receptor class C group 5 member A; anti-GPRC5A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-GPRC5A antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 307 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human GPRC5A by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GPRC5A antibody AAA212694 (lot AAA212694-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human GPRC5A by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GPRC5A antibody AAA212694 (lot AAA212694-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human GPRC5A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-GPRC5A antibody AAA212694 (lot AAA212694-1) used for IP at 6 ug per reaction. GPRC5A was also immunoprecipitated by rabbit anti-GPRC5A antibody For blotting immunoprecipitated GPRC5A, AAA212694 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human GPRC5A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-GPRC5A antibody AAA212694 (lot AAA212694-1) used for IP at 6 ug per reaction. GPRC5A was also immunoprecipitated by rabbit anti-GPRC5A antibody For blotting immunoprecipitated GPRC5A, AAA212694 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-GPRC5A antibody
G-protein coupled receptor family C group 5 member A (GPRC5A) is a member of the type 3 G protein-coupling receptor family, characterized by the signature 7-transmembrane domain motif. The encoded protein may be involved in interaction between retinoid acid and G protein signalling pathways. Retinoic acid plays a critical role in development, cellular growth, and differentiation. This gene may play a role in embryonic development and epithelial cell differentiation. [provided by RefSeq, Jul 2008] [taken from NCBI Entrez Gene (Gene ID: 9052)]. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8NFJ5].
Product Categories/Family for anti-GPRC5A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,251 Da
NCBI Official Full Name
retinoic acid-induced protein 3
NCBI Official Synonym Full Names
G protein-coupled receptor, family C, group 5, member A
NCBI Official Symbol
GPRC5A
NCBI Official Synonym Symbols
RAI3; RAIG1; GPCR5A
NCBI Protein Information
retinoic acid-induced protein 3; RAIG-1; retinoic acid induced 3; retinoic acid responsive; retinoic acid-induced gene 1 protein; orphan G-protein-coupling receptor PEIG-1; G-protein coupled receptor family C group 5 member A
UniProt Protein Name
Retinoic acid-induced protein 3
UniProt Gene Name
GPRC5A
UniProt Synonym Gene Names
GPCR5A; RAI3; RAIG1; RAIG-1
UniProt Entry Name
RAI3_HUMAN
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Product Notes
The GPRC5A gprc5a (Catalog #AAA212694) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-GPRC5A Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPRC5A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the GPRC5A gprc5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPRC5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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