Rabbit anti-Human Granzyme B Polyclonal Antibody | anti-GZMB antibody

Granzyme B

Cat. Num. AAA59221

• Gene Names: GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• Reactivity: Human
• Synonyms: Granzyme B, Antibody; Granzyme B; anti-GZMB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Granzyme B is a purified immunoglobulin fraction of rabbit antiserum that is filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

• Control: Tonsil, Lymph Node
• Localization: Cytoplasmic (Granular)
• Immunogen: Synthetic peptide corresponding to residues E(20) IIGGHEAKPHSRPYMAYL (38) of human Granzyme B.
• Reactivity Note: Paraffin, Frozen
• Precautions: 1.- For professional users only. Ensure results are interpreted by a medical professional. ; 2.- This product contains sodium azide (NAN₃,a toxic chemical which may react with this plumbing to form highly explosive build-ups o f metal azides. Upon dipsosal, flush with large volumes of water to prevent sodium azide build- up.; 3.-Ensure proper handling procedures are used with reagent. Alwayswear proper laboratory equipment such as laboratory coat and gloves when hangling reagents. ; 4.- Unused solution should be disposed of acording to local and federal regulations. ; 5.- Do not ingest reagent. If reagent ingested, contact poison control center inmediately,; 6.- For complete recommendations for hnadling biological specimens please refer to the CDC document, ''Guidelines for Safe Wrok Practives in Human and Animal Laboratories''.
• Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides ; 2. Air dry for 2 hours at 58° C. ; 3. Deparaffinize, dehydrate and rehydrate tissues. ; 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate . ; ; 5. Any of three heating methods may be used: ; ; a. TintoRetriever r Pressure Cooker or Equivalent; Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.; b. TintoRetriever PT Module or Water Bath Method ; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA ; Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.; c. Conventional Steamer Method; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA ; Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes. ; ; 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. ; 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. ; 8. Wash slides with IHC wash buffer or DI water. ; 9. Continue IHC staining protocol
• Preparation and Storage: Store at 2-8°C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropiately when not use, and avoid prolonged exposure to room temperature conditions.

IHC (Immunohistochemisry)

IHC (Immunohiostchemistry)

Application Data

Related Product Information for anti-GZMB antibody

Granzymes are exogenous serine proteases that are released by cytoplasmic granules within cytotoxic T-cells and natural killer cells. Their purpose is to induce apoptosis within virus-infected cells, thus destroying them. Anti-Granzyme B antibodies have been useful in identifying Natural Killer/T-cell Lymphoma, as well as Anaplastic Large Cell Lymphoma. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin's Disease.

NCBI and Uniprot Product Information

• NCBI GI #: 221625528
• NCBI GeneID: 3002
• NCBI Accession #: NP_004122.2
• NCBI GenBank Nucleotide #: NM_004131.4
• UniProt Accession #: P10144; Q8N1D2; Q9UCC1; Q67BC3; Q6XGZ2; Q6XGZ3; Q6XGZ4
• NCBI Official Full Name: granzyme B
• NCBI Official Synonym Full Names: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• NCBI Official Symbol: GZMB
• NCBI Official Synonym Symbols: HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• NCBI Protein Information: granzyme B; C11; CTLA-1; fragmentin 2; fragmentin-2; OTTHUMP00000178755; OTTHUMP00000228682; cathepsin G-like 1; human lymphocyte protein; T-cell serine protease 1-3E; cytotoxic serine protease B; cytotoxic T-lymphocyte proteinase 2
• UniProt Protein Name: Granzyme B
• UniProt Gene Name: GZMB
• UniProt Synonym Gene Names: CGL1; CSPB; CTLA1; GRB
• UniProt Entry Name: GRAB_HUMAN