Rabbit GRP78 Polyclonal Antibody | anti-HSPA5 antibody

GRP78 Antibody

Cat. Num. AAA286489

• Gene Names: HSPA5; BIP; MIF2; GRP78; HEL-S-89n
• Reactivity: Human, mouse, rat
• Applications: Immunohistochemistry, Immunofluorescence, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: GRP78, Antibody; GRP78 Antibody; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP; HSPA5; GRP78; anti-HSPA5 antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Positions: 623-654
• Sequence Length: 654

• Applicable Applications for anti-HSPA5 antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GRP78 antibody.)

IF (Immunofluorescence)

(Immunofluorescence analysis of COS7 cells, using GRP78 antibody.)

WB (Western Blot)

(Western blot analysis of extracts from HUVEC cells (Lane 1), COS7 cells (Lane 2) and JK cells (Lane 3), using GRP78 Antibody. The lane on the left is treated with systhesized peptide.)

WB (Western Blot)

(Western blot analysis of lysates from Hela,HepG2 cell line (from left to right),using GRP78 Antibody(C0217). C0217 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)

Related Product Information for anti-HSPA5 antibody

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

References

Ting J.,et al.DNA 7:275-286(1988).
Chao C.C.K.,et al.Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
Hansen J.J.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Bermudez-Fajardo A.,et al.Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
Humphray S.J.,et al.Nature 429:369-374(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 16507237
• NCBI GeneID: 3309
• NCBI Accession #: NP_005338.1
• NCBI GenBank Nucleotide #: NM_005347.4
• UniProt Accession #: P11021; Q2EF78; Q9NPF1; Q9UK02; B0QZ61
• Molecular Weight: 72333
• NCBI Official Full Name: 78 kDa glucose-regulated protein
• NCBI Official Synonym Full Names: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• NCBI Official Symbol: HSPA5
• NCBI Official Synonym Symbols: BIP; MIF2; GRP78; HEL-S-89n
• NCBI Protein Information: 78 kDa glucose-regulated protein
• UniProt Protein Name: 78 kDa glucose-regulated protein
• UniProt Gene Name: HSPA5
• UniProt Synonym Gene Names: GRP78; GRP-78; BiP
• UniProt Entry Name: GRP78_HUMAN