Application Data
(Grp78 antibody detects Grp78 protein by western blot analysis.A. 30 mug 293T whole cell lysate/extractB. 30 mug A431 whole cell lysate/extract C. 30 mug HeLa whole cell lysate/extractD. 30 mug HepG2 whole cell lysate/extract7.5 % SDS-PAGE #102567 dilution: 1:1000)
Rabbit anti-Human, Mouse Grp78 Polyclonal Antibody | anti-Grp78 antibody
Grp78 Antibody
Gene Names
HSPA5; BIP; MIF2; GRP78; HEL-S-89n
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Antibodies were purified by antigen-affinity chromatography.
Synonyms
Grp78, Antibody; Grp78 Antibody; BIP antibody; FLJ26106 antibody; GRP78 antibody; MIF2 antibody; HSPA5 antibody; immunoglobulin heavy chain-binding protein antibody; 78 kDa glucose-regulated protein antibody; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody; "heat shoc; anti-Grp78 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total Grp78 protein.
Purity/Purification
Antibodies were purified by antigen-affinity chromatography.
Form/Format
Rabbit IgG in 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
654
Applicable Applications for anti-Grp78 antibody
WB (Western Blot)
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant fragment corresponding to a region within amino acids 478 and 654 of Grp78.
Target Name
Grp78
Preparation and Storage
Store at -20 degree C
Application Data
(Grp78 antibody detects Grp78 protein by western blot analysis.A. 30 mug 293T whole cell lysate/extractB. 30 mug A431 whole cell lysate/extract C. 30 mug HeLa whole cell lysate/extractD. 30 mug HepG2 whole cell lysate/extract7.5 % SDS-PAGE #102567 dilution: 1:1000)
Application Data
(Grp78 antibody detects Grp78 protein by western blot analysis.A. 30 mug 293T whole cell lysate/extractB. 30 mug A431 whole cell lysate/extract C. 30 mug HeLa whole cell lysate/extractD. 30 mug HepG2 whole cell lysate/extract7.5 % SDS-PAGE #102567 dilution: 1:1000)
Application Data
(Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:2000)
Application Data
(Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:2000)
Related Product Information for anti-Grp78 antibody
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]
Product Categories/Family for anti-Grp78 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,333 Da
NCBI Official Full Name
78 kDa glucose-regulated protein
NCBI Official Synonym Full Names
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
NCBI Official Symbol
HSPA5
NCBI Official Synonym Symbols
BIP; MIF2; GRP78; HEL-S-89n
NCBI Protein Information
78 kDa glucose-regulated protein
UniProt Protein Name
78 kDa glucose-regulated protein
UniProt Gene Name
HSPA5
UniProt Synonym Gene Names
GRP78; GRP-78; BiP
UniProt Entry Name
GRP78_HUMAN
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Product Notes
The Grp78 hspa5 (Catalog #AAA306490) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Grp78 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Grp78 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the Grp78 hspa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Grp78, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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