Rabbit GSPT1 Polyclonal Antibody | anti-GSPT1 antibody

GSPT1 Antibody (N-term)

Cat. Num. AAA296704

• Reactivity: Human; Predicted reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: GSPT1, Antibody; GSPT1 Antibody (N-term); Eukaryotic peptide chain release factor GTP-binding subunit ERF3A; Eukaryotic peptide chain release factor subunit 3a; eRF3a; G1 to S phase transition protein 1 homolog; GSPT1; ERF3A; anti-GSPT1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone Number: RB24635
• Specificity: This GSPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-29 amino acids from the N-terminal region of human GSPT1.
• Purity/Purification: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

• Applicable Applications for anti-GSPT1 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
• Antigen Region: 2-29
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(GSPT1 Antibody (N-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(GSPT1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSPT1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(GSPT1 Antibody (N-term) western blot analysis in U251,CEM cell line lysates (35ug/lane).This demonstrates the GSPT1 antibody detected the GSPT1 protein (arrow).)

Related Product Information for anti-GSPT1 antibody

Involved in translation termination in response to the termination codons UAA, UAG and UGA (By similarity). Stimulates the activity of ETF1 (By similarity). Involved in regulation of mammalian cell growth (PubMed:2511002). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense- mediated decay (NMD) of mRNAs containing premature stop codons (PubMed:24486019). Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed:30682371).

Product Categories/Family for anti-GSPT1 antibody

Primary Antibodies

References

Malta-Vacas, J., et al. Cancer Genet. Cytogenet. 195(2):132-142(2009)Malta-Vacas, J., et al. Oncol. Rep. 21(6):1551-1558(2009)Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)Figaro, S., et al. FEBS Lett. 582(16):2352-2356(2008)Chauvin, C., et al. RNA 14(2):240-245(2008)

NCBI and Uniprot Product Information

• NCBI GeneID: 2935
• UniProt Accession #: P15170; Q8R050
• Molecular Weight: 55756Da
• UniProt Protein Name: Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
• UniProt Gene Name: GSPT1
• UniProt Synonym Gene Names: ERF3A; Eukaryotic peptide chain release factor subunit 3a; eRF3a
• UniProt Entry Name: ERF3A_HUMAN