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FCM/FACS (Flow Cytometry)
(GSR Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human GSR Polyclonal Antibody | anti-GSR antibody
GSR Antibody (C-term)
Gene Names
GSR; HEL-75
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GSR, Antibody; GSR Antibody (C-term); Glutathione reductase; mitochondrial; GR; GRase; GSR; GLUR; GRD1; anti-GSR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GSR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-408 amino acids from the C-terminal region of human GSR.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.33 (varies by lot)
Sequence Positions
380-408
Sequence Length
440
Applicable Applications for anti-GSR antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(GSR Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(GSR Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of GSR Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of GSR Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(GSR Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GSR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(GSR Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GSR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(GSR Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GSR antibody detected the GSR protein (arrow).)
WB (Western Blot)
(GSR Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GSR antibody detected the GSR protein (arrow).)
Related Product Information for anti-GSR antibody
This gene encodes a member of the class-I pyridine
nucleotide-disulfide oxidoreductase family. This enzyme is a
homodimeric flavoprotein. It is a central enzyme of cellular
antioxidant defense, and reduces oxidized glutathione disulfide
(GSSG) to the sulfhydryl form GSH, which is an important cellular
antioxidant. Rare mutations in this gene result in hereditary
glutathione reductase deficiency. Multiple alternatively spliced
transcript variants encoding different isoforms have been found.
nucleotide-disulfide oxidoreductase family. This enzyme is a
homodimeric flavoprotein. It is a central enzyme of cellular
antioxidant defense, and reduces oxidized glutathione disulfide
(GSSG) to the sulfhydryl form GSH, which is an important cellular
antioxidant. Rare mutations in this gene result in hereditary
glutathione reductase deficiency. Multiple alternatively spliced
transcript variants encoding different isoforms have been found.
Product Categories/Family for anti-GSR antibody
References
Satoh, N., et al. Biochem. Genet. 48 (9-10), 816-821 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Wang, Y., et al. J. Hum. Genet. 55(8):490-494(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Moyer, A.M., et al. Cancer Epidemiol. Biomarkers Prev. 19(3):811-821(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Wang, Y., et al. J. Hum. Genet. 55(8):490-494(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Moyer, A.M., et al. Cancer Epidemiol. Biomarkers Prev. 19(3):811-821(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56257
NCBI Official Full Name
glutathione reductase, mitochondrial isoform 1
NCBI Official Synonym Full Names
glutathione reductase
NCBI Official Symbol
GSR
NCBI Official Synonym Symbols
HEL-75
NCBI Protein Information
glutathione reductase, mitochondrial
UniProt Protein Name
Glutathione reductase, mitochondrial
UniProt Gene Name
GSR
UniProt Synonym Gene Names
GLUR; GRD1; GR; GRase
UniProt Entry Name
GSHR_HUMAN
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Product Notes
The GSR gsr (Catalog #AAA286150) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 380-408. The GSR Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GSR can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GSR gsr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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