Rabbit anti-Human GSTM5 Polyclonal Antibody | anti-GSTM5 antibody

GSTM5 Antibody (N-term)

Cat. Num. AAA287324

• Gene Names: GSTM5; GTM5; GSTM5-5
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: GSTM5, Antibody; GSTM5 Antibody (N-term); Glutathione S-transferase Mu 5; GST class-mu 5; GSTM5-5; GSTM5; anti-GSTM5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This GSTM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-43 amino acids from the N-terminal region of human GSTM5.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 14-43
• Sequence Length: 218

• Applicable Applications for anti-GSTM5 antibody: WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(GSTM5 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(GSTM5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GSTM5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of lysate from human brain tissue lysate, using GSTM5 Antibody (N-term). AAA287324 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)

Related Product Information for anti-GSTM5 antibody

Cytosolic and membrane-bound forms of glutathione
S-transferase are encoded by two distinct supergene families. At
present, eight distinct classes of the soluble cytoplasmic
mammalian glutathione S-transferases have been identified: alpha,
kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a
glutathione S-transferase that belongs to the mu class. The mu
class of enzymes functions in the detoxification of electrophilic
compounds, including carcinogens, therapeutic drugs, environmental
toxins and products of oxidative stress, by conjugation with
glutathione. The genes encoding the mu class of enzymes are
organized in a gene cluster on chromosome 1p13.3 and are known to
be highly polymorphic. These genetic variations can change an
individual's susceptibility to carcinogens and toxins as well as
affect the toxicity and efficacy of certain drugs. Diversification
of these genes has occurred in regions encoding substrate-binding
domains, as well as in tissue expression patterns, to accommodate
an increasing number of foreign compounds.

Product Categories/Family for anti-GSTM5 antibody

Metabolism; Signal Transduction

References

Wang, Y., et al. J. Hum. Genet. 55(8):490-494(2010)
Yu, K.D., et al. Breast Cancer Res. Treat. 121(2):485-496(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Moyer, A.M., et al. Cancer Epidemiol. Biomarkers Prev. 19(3):811-821(2010)
Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 23065563
• NCBI GeneID: 2949
• NCBI Accession #: NP_000842.2
• NCBI GenBank Nucleotide #: NM_000851.3
• UniProt Accession #: P46439; Q6PD78; A8K0V8
• Molecular Weight: 25675
• NCBI Official Full Name: glutathione S-transferase Mu 5
• NCBI Official Synonym Full Names: glutathione S-transferase mu 5
• NCBI Official Symbol: GSTM5
• NCBI Official Synonym Symbols: GTM5; GSTM5-5
• NCBI Protein Information: glutathione S-transferase Mu 5
• UniProt Protein Name: Glutathione S-transferase Mu 5
• UniProt Gene Name: GSTM5
• UniProt Entry Name: GSTM5_HUMAN