WB (Western Blot)
(Detection of human GTF2H1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GTF2H1 antibody AAA212201 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. GTF2H1 was also immunoprecipitated by rabbit anti-GTF2H1 antibody BL12538, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
Rabbit anti-Human GTF2H1 Polyclonal Antibody | anti-GTF2H1 antibody
Rabbit anti-GTF2H1 Antibody, Affinity Purified
Gene Names
GTF2H1; P62; BTF2; TFB1; TFIIH
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
GTF2H1, Antibody; Rabbit anti-GTF2H1 Antibody, Affinity Purified; general transcription factor IIH polypeptide 1; TFIIH; TFB1; P62; general transcription factor IIH subunit 1; TFIIH basal transcription factor complex p62 subunit; epididymis secretory sperm binding protein; BTF2 p62; BTF2; basic transcription factor 2 62 kDa subunit; general transcription factor IIH, polypeptide 1, 62kDa; General transcription factor IIH subunit 1; anti-GTF2H1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-GTF2H1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 250 and 300
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human GTF2H1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GTF2H1 antibody AAA212201 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. GTF2H1 was also immunoprecipitated by rabbit anti-GTF2H1 antibody BL12538, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human GTF2H1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-GTF2H1 antibody AAA212201 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. GTF2H1 was also immunoprecipitated by rabbit anti-GTF2H1 antibody BL12538, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human GTF2H1 by immunohistochemistry. Sample: FFPE section of human testis. Antibody: Affinity purified rabbit anti-GTF2H1 (Cat. No. AAA212201 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human GTF2H1 by immunohistochemistry. Sample: FFPE section of human testis. Antibody: Affinity purified rabbit anti-GTF2H1 (Cat. No. AAA212201 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-GTF2H1 antibody
GTF2H1 (general transcription factor IIH, polypeptide 1, 62kDa) is a component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA. When complexed to CAK, GTF2H1 is involved in RNA transcription by RNA polymerase II [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P32780].
Product Categories/Family for anti-GTF2H1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,032 Da
NCBI Official Full Name
general transcription factor IIH subunit 1
NCBI Official Synonym Full Names
general transcription factor IIH, polypeptide 1, 62kDa
NCBI Official Symbol
GTF2H1
NCBI Official Synonym Symbols
P62; BTF2; TFB1; TFIIH
NCBI Protein Information
general transcription factor IIH subunit 1; BTF2 p62; basic transcription factor 2 62 kDa subunit; general transcription factor IIH polypeptide 1; TFIIH basal transcription factor complex p62 subunit
UniProt Protein Name
General transcription factor IIH subunit 1
UniProt Gene Name
GTF2H1
UniProt Synonym Gene Names
BTF2; BTF2 p62
UniProt Entry Name
TF2H1_HUMAN
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Product Notes
The GTF2H1 gtf2h1 (Catalog #AAA212201) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-GTF2H1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GTF2H1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the GTF2H1 gtf2h1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF2H1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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