WB (Western Blot)
(Western blot analysis of GTF2H4 Antibody (Center) in Y79 cell line lysates (35ug/lane). GTF2H4 (arrow) was detected using the purified Pab.)
Rabbit GTF2H4 Polyclonal Antibody | anti-GTF2H4 antibody
GTF2H4 Antibody (Center)
Gene Names
GTF2H4; P52; TFB2; TFIIH
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
GTF2H4, Antibody; GTF2H4 Antibody (Center); General transcription factor IIH subunit 4; Basic transcription factor 2 52 kDa subunit; BTF2 p52; General transcription factor IIH polypeptide 4; TFIIH basal transcription factor complex p52 subunit; GTF2H4; anti-GTF2H4 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GTF2H4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-151 amino acids from the Central region of human GTF2H4.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
123-151
Sequence Length
462
Applicable Applications for anti-GTF2H4 antibody
ELISA, WB (Western Blot)
Function
Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.
Cellular Location
Nucleus.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of GTF2H4 Antibody (Center) in Y79 cell line lysates (35ug/lane). GTF2H4 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of GTF2H4 Antibody (Center) in Y79 cell line lysates (35ug/lane). GTF2H4 (arrow) was detected using the purified Pab.)
Related Product Information for anti-GTF2H4 antibody
Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.
References
Blau,J., et.al., Mol. Cell. Biol. 16 (5), 2044-2055 (1996)
Zhou,Q. et.al., Science 274 (5287), 605-610 (1996)
Zhou,Q. et.al., Science 274 (5287), 605-610 (1996)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52186
NCBI Official Full Name
general transcription factor IIH subunit 4
NCBI Official Synonym Full Names
general transcription factor IIH, polypeptide 4, 52kDa
NCBI Official Symbol
GTF2H4
NCBI Official Synonym Symbols
P52; TFB2; TFIIH
NCBI Protein Information
general transcription factor IIH subunit 4
UniProt Protein Name
General transcription factor IIH subunit 4
UniProt Gene Name
GTF2H4
UniProt Synonym Gene Names
BTF2 p52
UniProt Entry Name
TF2H4_HUMAN
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Product Notes
The GTF2H4 gtf2h4 (Catalog #AAA288689) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 123-151. The GTF2H4 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's GTF2H4 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GTF2H4 gtf2h4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF2H4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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