WB (Western Blot)
(GTF2I Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the GTF2I antibody detected the GTF2I protein (arrow).)
Rabbit GTF2I Polyclonal Antibody | anti-GTF2I antibody
GTF2I Antibody (N-term)
Gene Names
GTF2I; WBS; DIWS; SPIN; IB291; BAP135; BTKAP1; TFII-I; WBSCR6; GTFII-I
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GTF2I, Antibody; GTF2I Antibody (N-term); General transcription factor II-I; GTFII-I; TFII-I; Bruton tyrosine kinase-associated protein 135; BAP-135; BTK-associated protein 135; SRF-Phox1-interacting protein; SPIN; Williams-Beuren syndrome chromosomal region 6 protein; GTF2I; BAP135; WBSCR6; anti-GTF2I antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GTF2I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-310 amino acids from the N-terminal region of human GTF2I.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.4 (varies by lot)
Sequence Positions
281-310
Sequence Length
976
Applicable Applications for anti-GTF2I antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(GTF2I Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the GTF2I antibody detected the GTF2I protein (arrow).)
WB (Western Blot)
(GTF2I Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the GTF2I antibody detected the GTF2I protein (arrow).)
IF (Immunofluorescence)
(Fluorescent image of Hela cell stained with GTF2I Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). GTF2I immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent image of Hela cell stained with GTF2I Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). GTF2I immunoreactivity is localized to Nucleus significantly.)
Related Product Information for anti-GTF2I antibody
This gene encodes a multifunctional phosphoprotein with
roles in transcription and signal transduction. It is deleted in
Williams-Beuren syndrome, a multisystem developmental disorder
caused by the deletion of contiguous genes at chromosome 7q11.23.
Alternative splicing results in multiple transcript variants.
Related pseudogenes have been identified on chromosomes 7, 13 and
21.
roles in transcription and signal transduction. It is deleted in
Williams-Beuren syndrome, a multisystem developmental disorder
caused by the deletion of contiguous genes at chromosome 7q11.23.
Alternative splicing results in multiple transcript variants.
Related pseudogenes have been identified on chromosomes 7, 13 and
21.
Product Categories/Family for anti-GTF2I antibody
References
Antonell, A., et al. J. Med. Genet. 47(5):312-320(2010)
Lazebnik, M.B., et al. J. Biol. Chem. 284(52):36234-36239(2009)
Sacristan, C., et al. Eur. J. Immunol. 39(9):2584-2595(2009)
Makeyev, A.V., et al. Gene 433 (1-2), 16-25 (2009) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Lazebnik, M.B., et al. J. Biol. Chem. 284(52):36234-36239(2009)
Sacristan, C., et al. Eur. J. Immunol. 39(9):2584-2595(2009)
Makeyev, A.V., et al. Gene 433 (1-2), 16-25 (2009) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
112416
NCBI Official Full Name
general transcription factor II-I isoform 5
NCBI Official Synonym Full Names
general transcription factor IIi
NCBI Official Symbol
GTF2I
NCBI Official Synonym Symbols
WBS; DIWS; SPIN; IB291; BAP135; BTKAP1; TFII-I; WBSCR6; GTFII-I
NCBI Protein Information
general transcription factor II-I
UniProt Protein Name
General transcription factor II-I
UniProt Gene Name
GTF2I
UniProt Synonym Gene Names
BAP135; WBSCR6; GTFII-I; TFII-I; BAP-135; BTK-associated protein 135; SPIN
UniProt Entry Name
GTF2I_HUMAN
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Product Notes
The GTF2I gtf2i (Catalog #AAA286306) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 281-310. The GTF2I Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's GTF2I can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GTF2I gtf2i for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF2I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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