WB (Western Blot)
(Detection of human GTF3C3/TFIIIC102 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GTF3C3/TFIIIC102 antibody AAA211371 (lot AAA211371-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human GTF3C3/TFIIIC102 Polyclonal Antibody | anti-GTF3C3/TFIIIC102 antibody
Rabbit anti-GTF3C3/TFIIIC102 Antibody, Affinity Purified
Gene Names
GTF3C3; TFIIIC102; TFIIICgamma; TFiiiC2-102
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
GTF3C3/TFIIIC102, Antibody; Rabbit anti-GTF3C3/TFIIIC102 Antibody, Affinity Purified; TFIIIC 102 kDa subunit; transcription factor IIIC subunit gamma; Transcription factor IIIC 102 kDa subunit; TFIIICgamma; TFIIIC102; TF3C-gamma; general transcription factor IIIC, polypeptide 3, 102kDa; general transcription factor 3C polypeptide 3; TFiiiC2-102; General transcription factor 3C polypeptide 3; general transcription factor IIIC subunit 3; anti-GTF3C3/TFIIIC102 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-GTF3C3/TFIIIC102 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 500 and 550
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human GTF3C3/TFIIIC102 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GTF3C3/TFIIIC102 antibody AAA211371 (lot AAA211371-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human GTF3C3/TFIIIC102 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GTF3C3/TFIIIC102 antibody AAA211371 (lot AAA211371-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human GTF3C3/TFIIIC102 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GTF3C3/TFIIIC102 antibody AAA211371 (lot AAA211371-2) used for IP at 3 ug per reaction. GTF3C3/TFIIIC102 was also immunoprecipitated by a previous lot of this antibody (lot AAA211371-1). For blotting immunoprecipitated GTF3C3/TFIIIC102, AAA211371 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human GTF3C3/TFIIIC102 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GTF3C3/TFIIIC102 antibody AAA211371 (lot AAA211371-2) used for IP at 3 ug per reaction. GTF3C3/TFIIIC102 was also immunoprecipitated by a previous lot of this antibody (lot AAA211371-1). For blotting immunoprecipitated GTF3C3/TFIIIC102, AAA211371 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human GTF3C3/TFIIIC102 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GTF3C3/TFIIIC102 (Cat. No. AAA211371 Lot2) used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human GTF3C3/TFIIIC102 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GTF3C3/TFIIIC102 (Cat. No. AAA211371 Lot2) used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-GTF3C3/TFIIIC102 antibody
General transcription factor 3C polypeptide 3 (GTF3C3/TFIIIC102) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. GTF3C3/TFIIIC102 appears to be important for stable complex formation and recruitment of RNA polymerase III by interacting with TFIIIB90, TBP, and TFIIIC63. GTF3C3/TFIIIC102 has been shown to also interact with DEDD and FLAME-3 proteins. This interaction may function to translocate GTF3C3/TFIIIC102 to the nucleus and regulate TFIIIC activity.
Product Categories/Family for anti-GTF3C3/TFIIIC102 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,258 Da
NCBI Official Full Name
general transcription factor 3C polypeptide 3 isoform 1
NCBI Official Synonym Full Names
general transcription factor IIIC, polypeptide 3, 102kDa
NCBI Official Symbol
GTF3C3
NCBI Official Synonym Symbols
TFIIIC102; TFIIICgamma; TFiiiC2-102
NCBI Protein Information
general transcription factor 3C polypeptide 3; TF3C-gamma; TFIIIC 102 kDa subunit; transcription factor IIIC subunit gamma
UniProt Protein Name
General transcription factor 3C polypeptide 3
UniProt Gene Name
GTF3C3
UniProt Synonym Gene Names
TFIIIC 102 kDa subunit; TFIIIC102; TF3C-gamma
UniProt Entry Name
TF3C3_HUMAN
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Product Notes
The GTF3C3/TFIIIC102 gtf3c3 (Catalog #AAA211371) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-GTF3C3/TFIIIC102 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GTF3C3/TFIIIC102 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the GTF3C3/TFIIIC102 gtf3c3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF3C3/TFIIIC102, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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