Rabbit anti-Human, mouse GZMB Polyclonal Antibody | anti-GZMB antibody

GZMB Antibody (N-term)

Cat. Num. AAA289186

• Gene Names: GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• Reactivity: Human, mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: GZMB, Antibody; GZMB Antibody (N-term); Granzyme B; C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E; GZMB; CGL1; CSPB; CTLA1; GRB; anti-GZMB antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This GZMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human GZMB.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 2-32
• Sequence Length: 247

• Applicable Applications for anti-GZMB antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Abundant protease in the cytosolic granules of cytotoxic T- cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:3262682, PubMed:3263427, PubMed:1985927). It cleaves after Asp (PubMed:8258716, PubMed:1985927). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore- forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed:32188940, PubMed:31953257). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis (PubMed:9852092).
• Cellular Location: Secreted. Cytolytic granule. Note=Delivered into the target cell by perforin (PubMed:20038786).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(GZMB Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemisry)

(GZMB Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human Lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GZMB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of GZMB (arrow) using rabbit polyclonal GZMB Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.)

WB (Western Blot)

(Western blot analysis of GZMB antibody (N-term) in mouse stomach lysates (35ug/lane). GZMB (arrow) was detected using the purified Pab.)

Related Product Information for anti-GZMB antibody

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.

Product Categories/Family for anti-GZMB antibody

Cancer; Cell Biology; Immunology; Signal Transduction

References

Hagn,M., J. Immunol. 183 (3), 1838-1845 (2009)
Gaafar,A., Exp. Hematol. 37 (7), 838-848 (2009)
Girnita,D.M., Transplantation 87 (12), 1801-1806 (2009)

NCBI and Uniprot Product Information

• NCBI GI #: 221625528
• NCBI GeneID: 3002
• NCBI Accession #: NP_004122.2
• NCBI GenBank Nucleotide #: NM_004131.4
• UniProt Accession #: P10144; Q8N1D2; Q9UCC1
• Molecular Weight: 27716
• NCBI Official Full Name: granzyme B
• NCBI Official Synonym Full Names: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• NCBI Official Symbol: GZMB
• NCBI Official Synonym Symbols: HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
• NCBI Protein Information: granzyme B
• UniProt Protein Name: Granzyme B
• UniProt Gene Name: GZMB
• UniProt Synonym Gene Names: CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP
• UniProt Entry Name: GRAB_HUMAN