IP (Immunoprecipitation)
(Mouse CDH13 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate0.5 uL anti-Mouse CDH13 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CDH13 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 78 kDaObserved band size: 103 kDa)
Rabbit anti-Mouse H Cadherin Polyclonal Antibody | anti-CDH13 antibody
Anti-H Cadherin Antibody, Rabbit Polyclonal
Gene Names
Cdh13; Cdht; Tcad; T-cadherin; 4932416G01Rik
Reactivity
Mouse
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
H Cadherin, Antibody; Anti-H Cadherin Antibody, Rabbit Polyclonal; CDH13/Cadherin-13/H Cadherin Antibody, Rabbit PAb, Antigen Affinity Purified; cadherin 13; Anti-4932416G01Rik Antibody; Anti-Cdht Antibody; Anti-T-cadherin Antibody; Anti-Tcad Antibody; anti-CDH13 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse CDH13/Cadherin-13/H Cadherin
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CDH13 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Mouse CDH13/Cadherin-13/H Cadherin protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CDH13/Cadherin-13/H Cadherin. CDH13/Cadherin-13/H Cadherin specific IgG was purified by Mouse CDH13/Cadherin-13/H Cadherin affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(Mouse CDH13 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate0.5 uL anti-Mouse CDH13 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CDH13 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 78 kDaObserved band size: 103 kDa)
IP (Immunoprecipitation)
(Mouse CDH13 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate0.5 uL anti-Mouse CDH13 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CDH13 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 78 kDaObserved band size: 103 kDa)
WB (Western Blot)
(Anti-CDH13 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:78 kDaObserved band size:85 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-CDH13 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:78 kDaObserved band size:85 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-CDH13 antibody
CDH13, also known as cadherin-13 and H Cadherin, is a member of the cadherin superfamily. CDH13 acts as a negative regulator of axon growth during neural differentiation. It also protects vascular endothelial cells from apoptosis due to oxidative stress, and is associated with resistance to atherosclerosis. CDH13 is localized to the surface of the cell membrane and is anchored by a GPI moiety, rather than by a transmembrane domain. CDH13 gene is hypermethylated in many types of cancer.
Product Categories/Family for anti-CDH13 antibody
References
Hart AB. et al., 2012, PLoS One. 7 (8): e42646. Xu J. et al., 2012, BMC Cancer. 12: 243. Jo J. et al., 2012, Obesity (Silver Spring). 20 (8): 1683-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
78,186 Da
NCBI Official Full Name
cadherin-13
NCBI Official Synonym Full Names
cadherin 13
NCBI Official Symbol
Cdh13
NCBI Official Synonym Symbols
Cdht; Tcad; T-cadherin; 4932416G01Rik
NCBI Protein Information
cadherin-13
UniProt Protein Name
Cadherin-13
UniProt Gene Name
Cdh13
UniProt Synonym Gene Names
H-cadherin; T-cad; T-cadherin
UniProt Entry Name
CAD13_MOUSE
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Product Notes
The CDH13 cdh13 (Catalog #AAA257261) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-H Cadherin Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's H Cadherin can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CDH13 cdh13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H Cadherin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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