WB (Western Blot)
(Detection of human and mouse H1F0/Histone H1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-H1F0/Histone H1.0 antibody (AAA213061 lot 1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse H1F0/Histone H1.0 Polyclonal Antibody | anti-H1F0/HistoneH1.0 antibody
Rabbit anti-H1F0/Histone H1.0 Antibody, Affinity Purified
Gene Names
H1F0; H10; H1FV
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
H1F0/Histone H1.0, Antibody; Rabbit anti-H1F0/Histone H1.0 Antibody, Affinity Purified; H1FV; histone H1.0; histone H1'; H10; H1.0, H1(0), H1-0; histone H1(0); Histone H1.0; H1 histone family member 0; anti-H1F0/HistoneH1.0 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-H1F0/HistoneH1.0 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 75 and 125
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse H1F0/Histone H1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-H1F0/Histone H1.0 antibody (AAA213061 lot 1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse H1F0/Histone H1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-H1F0/Histone H1.0 antibody (AAA213061 lot 1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human H1F0/Histone H1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-H1F0/Histone H1.0 antibody AAA213061 (lot AAA213061-1) used for IP at 6 ug per reaction. H1F0/Histone H1.0 was also immunoprecipitated by rabbit anti-H1F0/Histone H1.0 antibody BL19884. For blotting immunoprecipitated H1F0/Histone H1.0, AAA213061 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human H1F0/Histone H1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-H1F0/Histone H1.0 antibody AAA213061 (lot AAA213061-1) used for IP at 6 ug per reaction. H1F0/Histone H1.0 was also immunoprecipitated by rabbit anti-H1F0/Histone H1.0 antibody BL19884. For blotting immunoprecipitated H1F0/Histone H1.0, AAA213061 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-H1F0/HistoneH1.0 antibody
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P07305].
Product Categories/Family for anti-H1F0/HistoneH1.0 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,863 Da
NCBI Official Full Name
histone H1.0
NCBI Official Synonym Full Names
H1 histone family, member 0
NCBI Official Symbol
H1F0
NCBI Official Synonym Symbols
H10; H1FV
NCBI Protein Information
histone H1.0; histone H1'; histone H1(0); H1.0, H1(0), H1-0
UniProt Protein Name
Histone H1.0
UniProt Gene Name
H1F0
UniProt Synonym Gene Names
H1FV
UniProt Entry Name
H10_HUMAN
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Product Notes
The H1F0/HistoneH1.0 h1f0 (Catalog #AAA213061) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-H1F0/Histone H1.0 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's H1F0/Histone H1.0 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the H1F0/HistoneH1.0 h1f0 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H1F0/Histone H1.0, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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