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ChIP (Chromatin Immunoprecipitation)
(ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 provided a control(Provided by Shucai Wang, Su-Hwan Kwak:Development 134, 3873-3882 (2007)))
Rabbit HA Tag Polyclonal Antibody
HA Tag Antibody
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunofluorescence, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HA Tag, Antibody; HA Tag Antibody; Glutathione S-transferase; anti-HA Tag antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Applicable Applications for anti-HA Tag antibody
ChIP (Chromatin immunoprecipitation), IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
ChIP (Chromatin Immunoprecipitation)
(ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 provided a control(Provided by Shucai Wang, Su-Hwan Kwak:Development 134, 3873-3882 (2007)))
ChIP (Chromatin Immunoprecipitation)
(ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 provided a control(Provided by Shucai Wang, Su-Hwan Kwak:Development 134, 3873-3882 (2007)))
IF (Immunofluorescence)
(GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko:Journal of Cell Science 120, 3188-3199 (2007)))
IF (Immunofluorescence)
(GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko:Journal of Cell Science 120, 3188-3199 (2007)))
WB (Western Blot)
(Western blot analysis of anti-HA tag pab in HA-tagged recombinant protein bacterial lysate.)
WB (Western Blot)
(Western blot analysis of anti-HA tag pab in HA-tagged recombinant protein bacterial lysate.)
WB (Western Blot)
(Western blot analysis of lysate from 12 tag recombinant protein cell line, using Tag-HA. 2x Antibody. AAA289184 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
WB (Western Blot)
(Western blot analysis of lysate from 12 tag recombinant protein cell line, using Tag-HA. 2x Antibody. AAA289184 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Related Product Information for anti-HA Tag antibody
Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. AAA Biotech's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.
Product Categories/Family for anti-HA Tag antibody
References
Marco. et.al., J Biol Chem. (2009) February 6; 284(6): 3577 585. Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. Sells MA and Chernoff J. (1995) Gene, 152:187-9.
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Product Notes
The HA Tag (Catalog #AAA289184) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HA Tag can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HA Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HA Tag, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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