<
IP (Immunoprecipitation)
(HADH was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-HADH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HADH rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :34 kDa)
Rabbit anti-Human HADH Polyclonal Antibody | anti-HADH antibody
Anti-HADH Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
HADH, Antibody; Anti-HADH Antibody, Rabbit Polyclonal; HADH Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-HAD Antibody; Anti-HADH1 Antibody; Anti-HADHSC Antibody; Anti-HCDH Antibody; Anti-HHF4 Antibody; Anti-MSCHAD Antibody; Anti-SCHAD Antibody; hydroxyacyl-CoA dehydrogenase; anti-HADH antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human HADH
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid. PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-HADH antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human HADH fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human HADH fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
IP (Immunoprecipitation)
(HADH was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-HADH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HADH rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :34 kDa)
IP (Immunoprecipitation)
(HADH was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-HADH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HADH rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size :34 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of HADH in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human HADH polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor-488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of HADH in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human HADH polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor-488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)
IHC (Immunohistochemisry)
(Immunochemical staining of human HADH in human liver with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining of human HADH in human liver with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human HADH in human kidney with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human HADH in human kidney with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-HADH rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: A431 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa)
WB (Western Blot)
(Anti-HADH rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: A431 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa)
Related Product Information for anti-HADH antibody
Background: HADH (Hydroxyacyl-CoA Dehydrogenase, also known as HADHSC) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. HADH is a key enzyme in fatty acid oxidation. It is widely expressed in fat, kidney, and other tissues. The downregulation of HADH promotes gastric cancer progression via activation of the Akt signaling pathway. Recessive mutations in the hydroxyacyl-CoA dehydrogenase (HADH) gene encoding the enzyme 3-hydroxyacyl-CoA dehydrogenase are a rare cause of diazoxide-responsive hyperinsulinemic hypoglycemia (HH).
Similar Products
Product Notes
The HADH (Catalog #AAA257629) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HADH Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HADH can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HADH for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HADH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.