FCM/FACS (Flow Cytometry)
(HADHB Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit HADHB Polyclonal Antibody | anti-HADHB antibody
HADHB Antibody (C-term)
Gene Names
HADHB; ECHB; MTPB; MSTP029; TP-BETA
Reactivity
Human (Predicted Reactivity: Bovine, Monkey)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HADHB, Antibody; HADHB Antibody (C-term); Trifunctional enzyme subunit beta; mitochondrial; TP-beta; 3-ketoacyl-CoA thiolase; Acetyl-CoA acyltransferase; Beta-ketothiolase; HADHB; anti-HADHB antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Monkey)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HADHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-342 amino acids from the C-terminal region of human HADHB.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.4 (varies by lot)
Sequence Positions
315-342
Sequence Length
474
Applicable Applications for anti-HADHB antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(HADHB Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(HADHB Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of HADHB Antibody (C-term) in Jurkat cell line lysates (35ug/lane). HADHB (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of HADHB Antibody (C-term) in Jurkat cell line lysates (35ug/lane). HADHB (arrow) was detected using the purified Pab.)
Related Product Information for anti-HADHB antibody
HADHB encodes the beta subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the beta subunit catalyzing the 3-ketoacyl-CoA thiolase activity. Mutations in this gene result in trifunctional protein deficiency. The encoded protein can also bind RNA and decreases the stability of some mRNAs. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.
Product Categories/Family for anti-HADHB antibody
References
Purevsuren, J., et al. Mol. Genet. Metab. 98(4):372-377(2009)
Bogenhagen, D.F., et al. J. Biol. Chem. 283(6):3665-3675(2008)
Wang, R., et al. Zhonghua Fu Chan Ke Za Zhi 41(10):672-675(2006)
Hillier, L.W., et al. Nature 434(7034):724-731(2005)
Bogenhagen, D.F., et al. J. Biol. Chem. 283(6):3665-3675(2008)
Wang, R., et al. Zhonghua Fu Chan Ke Za Zhi 41(10):672-675(2006)
Hillier, L.W., et al. Nature 434(7034):724-731(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51294
NCBI Official Full Name
trifunctional enzyme subunit beta, mitochondrial isoform 1
NCBI Official Synonym Full Names
hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit
NCBI Official Symbol
HADHB
NCBI Official Synonym Symbols
ECHB; MTPB; MSTP029; TP-BETA
NCBI Protein Information
trifunctional enzyme subunit beta, mitochondrial
UniProt Protein Name
Trifunctional enzyme subunit beta, mitochondrial
UniProt Gene Name
HADHB
UniProt Entry Name
ECHB_HUMAN
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Product Notes
The HADHB hadhb (Catalog #AAA289182) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 315-342. The HADHB Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's HADHB can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HADHB hadhb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HADHB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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