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IHC (Immunohistochemistry)
(Immunohistochemistry of paraffinembedded human tonsil using HCAR2 Rabbit pAb (AAA28288) at dilution of 1:25 (40x lens).)
Rabbit HCAR2 Polyclonal Antibody | anti-HCAR2 antibody
HCAR2 Polyclonal Antibody
Gene Names
HCAR2; HCA2; HM74a; HM74b; PUMAG; NIACR1; Puma-g; GPR109A
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Purification
Synonyms
HCAR2, Antibody; HCAR2 Polyclonal Antibody; HCAR2; GPR109A; HCA2; HM74a; HM74b; NIACR1; PUMAG; Puma-g; hydroxycarboxylic acid receptor 2; anti-HCAR2 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-HCAR2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:50-1:200
IHC: 1:50-1:200
IF/ICC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human GPR109A/HM74A/HCAR2 (NP_808219.1)
Species Reactivity/Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at -20°C. Avoid freeze/thaw cycles.
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffinembedded human tonsil using HCAR2 Rabbit pAb (AAA28288) at dilution of 1:25 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffinembedded human tonsil using HCAR2 Rabbit pAb (AAA28288) at dilution of 1:25 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using HCAR2 antibody (AAA28288) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using HCAR2 antibody (AAA28288) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using HCAR2 antibody (AAA28288) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using HCAR2 antibody (AAA28288) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using HCAR2 antibody (AAA28288) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using HCAR2 antibody (AAA28288) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Related Product Information for anti-HCAR2 antibody
Acts as a high affinity receptor for both nicotinic acid (also known as niacin and (D-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed MW: 44KDa
Calculated MW: 42kDa
Calculated MW: 42kDa
NCBI Official Full Name
G protein-coupled receptor 109A
NCBI Official Synonym Full Names
hydroxycarboxylic acid receptor 2
NCBI Official Symbol
HCAR2
NCBI Official Synonym Symbols
HCA2; HM74a; HM74b; PUMAG; NIACR1; Puma-g; GPR109A
NCBI Protein Information
hydroxycarboxylic acid receptor 2
UniProt Protein Name
Hydroxycarboxylic acid receptor 2
UniProt Gene Name
HCAR2
UniProt Synonym Gene Names
GPR109A; HCA2; HM74A; NIACR1
Similar Products
Product Notes
The HCAR2 hcar2 (Catalog #AAA28288) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HCAR2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC). WB: 1:500-1:2000 IF/ICC: 1:50-1:200 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the HCAR2 hcar2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HCAR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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