FCM/FACS (Flow Cytometry)
(HCK Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit HCK Polyclonal Antibody | anti-HCK antibody
HCK Antibody (N-term)
Gene Names
HCK; JTK9; p59Hck; p61Hck
Reactivity
Human (Predicted Reactivity: Monkey, Mouse, Rat)
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HCK, Antibody; HCK Antibody (N-term); Tyrosine-protein kinase HCK; Hematopoietic cell kinase; Hemopoietic cell kinase; p59-HCK/p60-HCK; p59Hck; p61Hck; HCK; anti-HCK antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Monkey, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-156 amino acids from the N-terminal region of human HCK.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
131-156
Sequence Length
525
Applicable Applications for anti-HCK antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(HCK Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(HCK Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(HCK Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HCK Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(HCK Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HCK Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(HCK Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HCK antibody detected the HCK protein (arrow).)
WB (Western Blot)
(HCK Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HCK antibody detected the HCK protein (arrow).)
Related Product Information for anti-HCK antibody
HCK is a member of the Src
family of tyrosine kinases. This protein is primarily hemopoietic,
particularly in cells of the myeloid and B-lymphoid lineages. It
may help couple the Fc receptor to the activation of the
respiratory burst. In addition, it may play a role in neutrophil
migration and in the degranulation of neutrophils. Multiple
isoforms with different subcellular distributions are produced due
to both alternative splicing and the use of alternative translation
initiation codons, including a non-AUG (CUG) codon. [provided by
RefSeq].
family of tyrosine kinases. This protein is primarily hemopoietic,
particularly in cells of the myeloid and B-lymphoid lineages. It
may help couple the Fc receptor to the activation of the
respiratory burst. In addition, it may play a role in neutrophil
migration and in the degranulation of neutrophils. Multiple
isoforms with different subcellular distributions are produced due
to both alternative splicing and the use of alternative translation
initiation codons, including a non-AUG (CUG) codon. [provided by
RefSeq].
Product Categories/Family for anti-HCK antibody
References
Hassan, R., et al. J. Cell. Physiol. 221(2):458-468(2009)
Kennah, E., et al. Blood 113(19):4646-4655(2009)
Voss, M., et al. BMC Immunol. 10, 53 (2009) :
Rikova, K., et al. Cell 131(6):1190-1203(2007)
Kennah, E., et al. Blood 113(19):4646-4655(2009)
Voss, M., et al. BMC Immunol. 10, 53 (2009) :
Rikova, K., et al. Cell 131(6):1190-1203(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59600
NCBI Official Full Name
tyrosine-protein kinase HCK isoform b
NCBI Official Synonym Full Names
HCK proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol
HCK
NCBI Official Synonym Symbols
JTK9; p59Hck; p61Hck
NCBI Protein Information
tyrosine-protein kinase HCK
UniProt Protein Name
Tyrosine-protein kinase HCK
UniProt Gene Name
HCK
UniProt Entry Name
HCK_HUMAN
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Product Notes
The HCK hck (Catalog #AAA286199) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 131-156. The HCK Antibody (N-term) reacts with Human (Predicted Reactivity: Monkey, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's HCK can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the HCK hck for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HCK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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