IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with HDAC4 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC4 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HDAC4 immunoreactivity is localized to Nucleus significantly.)
Rabbit anti-Human HDAC4 Polyclonal Antibody | anti-HDAC4 antibody
HDAC4 Antibody (C-term)
Gene Names
HDAC4; HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HDAC4, Antibody; HDAC4 Antibody (C-term); Histone deacetylase 4; HD4; HDAC4; KIAA0288; anti-HDAC4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HDAC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1049-1077 amino acids from the C-terminal region of human HDAC4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.35 (varies by lot)
Sequence Positions
1049-1077
Sequence Length
972
Applicable Applications for anti-HDAC4 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with HDAC4 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC4 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HDAC4 immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with HDAC4 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC4 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HDAC4 immunoreactivity is localized to Nucleus significantly.)
IHC (Immunohiostchemistry)
(HDAC4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HDAC4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(HDAC4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HDAC4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(HDAC4 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the HDAC4 antibody detected the HDAC4 protein (arrow).)
WB (Western Blot)
(HDAC4 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the HDAC4 antibody detected the HDAC4 protein (arrow).)
Related Product Information for anti-HDAC4 antibody
Histones play a critical role in transcriptional
regulation, cell cycle progression, and developmental events.
Histone acetylation/deacetylation alters chromosome structure and
affects transcription factor access to DNA. The protein encoded by
this gene belongs to class II of the histone deacetylase/acuc/apha
family. It possesses histone deacetylase activity and represses
transcription when tethered to a promoter. This protein does not
bind DNA directly, but through transcription factors MEF2C and
MEF2D. It seems to interact in a multiprotein complex with RbAp48
and HDAC3.
regulation, cell cycle progression, and developmental events.
Histone acetylation/deacetylation alters chromosome structure and
affects transcription factor access to DNA. The protein encoded by
this gene belongs to class II of the histone deacetylase/acuc/apha
family. It possesses histone deacetylase activity and represses
transcription when tethered to a promoter. This protein does not
bind DNA directly, but through transcription factors MEF2C and
MEF2D. It seems to interact in a multiprotein complex with RbAp48
and HDAC3.
Product Categories/Family for anti-HDAC4 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Williams, S.R., et al. Am. J. Hum. Genet. 87(2):219-228(2010)
Kim, T., et al. Psychiatry Res 178(2):266-269(2010)
Pan, L., et al. Cell. Mol. Immunol. 7(3):221-226(2010)
Smith, J.A., et al. Virol. J. 7, 237 (2010) :
Williams, S.R., et al. Am. J. Hum. Genet. 87(2):219-228(2010)
Kim, T., et al. Psychiatry Res 178(2):266-269(2010)
Pan, L., et al. Cell. Mol. Immunol. 7(3):221-226(2010)
Smith, J.A., et al. Virol. J. 7, 237 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
119040
NCBI Official Full Name
histone deacetylase 4
NCBI Official Synonym Full Names
histone deacetylase 4
NCBI Official Symbol
HDAC4
NCBI Official Synonym Symbols
HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
NCBI Protein Information
histone deacetylase 4
UniProt Protein Name
Histone deacetylase 4
UniProt Gene Name
HDAC4
UniProt Synonym Gene Names
KIAA0288; HD4
UniProt Entry Name
HDAC4_HUMAN
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Product Notes
The HDAC4 hdac4 (Catalog #AAA283858) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1049-1077. The HDAC4 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the HDAC4 hdac4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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