Rabbit HDAC5 Polyclonal Antibody | anti-HDAC5 antibody

Rabbit polyclonal to Human HDAC5

Cat. Num. AAA10627

• Gene Names: HDAC5; HD5; NY-CO-9
• Reactivity: Human, mouse, rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: HDAC5, Antibody; Rabbit polyclonal to Human HDAC5; HDAC5 Rabbit pab Antibody; anti-HDAC5 antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1123

• Applicable Applications for anti-HDAC5 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1/500-1000; IHC: 1/50
• Immunogen: A synthetic peptide of human HDAC5
• Storage Buffer: PBS with 0.1% Sodium Azide, 50% Glycerol
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C or -80 degree C

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded mouse brain using HDAC5 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded mouse spinal cord using HDAC5 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded mouse testis using HDAC5 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded human breast cancer using HDAC5 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human tonsil using HDAC5 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded rat liver using HDAC5 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded mouse lung using HDAC5 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human mammary gland using HDAC5 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using HDAC5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Related Product Information for anti-HDAC5 antibody

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

References

1. Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703.
2. Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202.
3. Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53.
4. Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16.
5. Gray, S.G. and Ekström, T.J. (2001) Exp Cell Res 262, 75-83.

NCBI and Uniprot Product Information

• NCBI GI #: 62750349
• NCBI GeneID: 10014
• NCBI Accession #: NP_001015053.1
• NCBI GenBank Nucleotide #: NM_001015053.1
• UniProt Accession #: Q9UQL6; O60340; O60528; Q96DY4; C9JFV9
• Molecular Weight: 121,978 Da
• NCBI Official Full Name: histone deacetylase 5 isoform 3
• NCBI Official Synonym Full Names: histone deacetylase 5
• NCBI Official Symbol: HDAC5
• NCBI Official Synonym Symbols: HD5; NY-CO-9
• NCBI Protein Information: histone deacetylase 5; antigen NY-CO-9
• UniProt Protein Name: Histone deacetylase 5
• UniProt Gene Name: HDAC5
• UniProt Synonym Gene Names: KIAA0600; HD5
• UniProt Entry Name: HDAC5_HUMAN