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IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit HDAC9 Polyclonal Antibody | anti-HDAC9 antibody
HDAC9 Antibody (N-term)
Gene Names
HDAC9; HD7; HD9; HD7b; HDAC; HDRP; MITR; HDAC7; HDAC7B; HDAC9B; HDAC9FL
Reactivity
Human (Predicted Reactivity: Chicken, Mouse)
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, ELISA, Immunoprecipitation
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HDAC9, Antibody; HDAC9 Antibody (N-term); Histone deacetylase 9; HD9; Histone deacetylase 7B; HD7; HD7b; Histone deacetylase-related protein; MEF2-interacting transcription repressor MITR; HDAC9; HDAC7; HDAC7B; HDRP; KIAA0744; MITR; anti-HDAC9 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Chicken, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HDAC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human HDAC9.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
2-32
Sequence Length
513
Applicable Applications for anti-HDAC9 antibody
IF (Immunofluorescence), WB (Western Blot), IHC (Immunohistochemistry), ELISA, IP (Immunoprecipitation)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IF (Immunofluorescence)
(Figure 2: Immunofluorescence staining of MITR for a compartmentalization study in undifferentiated C2C12 myoblasts transfected with a MITR-expressing plasmid. MITR is detected by using the HDAC9 N-term antibody (top panel) or a DYKDDDDK antibody (bottom panel) detecting a DYKDDDDK epitope fused at the N-term end of the MITR construct. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.)
IF (Immunofluorescence)
(Figure 2: Immunofluorescence staining of MITR for a compartmentalization study in undifferentiated C2C12 myoblasts transfected with a MITR-expressing plasmid. MITR is detected by using the HDAC9 N-term antibody (top panel) or a DYKDDDDK antibody (bottom panel) detecting a DYKDDDDK epitope fused at the N-term end of the MITR construct. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.)
WB (Western Blot)
(Figure 1: Immunoblots for MITR (AAA284307 HDAC9 N-term antibody), Mirk, MyoD and tubulin proteins are shown for cytoplasmic (Cyt) and nuclear (N) extracts from undifferentiated C2C12 myoblasts. Before cell collection for fractionation, the cells are transfected with plasmids coding for Mirk (Wt), kinase-inactive Mirk (YF) or MITR. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.)
WB (Western Blot)
(Figure 1: Immunoblots for MITR (AAA284307 HDAC9 N-term antibody), Mirk, MyoD and tubulin proteins are shown for cytoplasmic (Cyt) and nuclear (N) extracts from undifferentiated C2C12 myoblasts. Before cell collection for fractionation, the cells are transfected with plasmids coding for Mirk (Wt), kinase-inactive Mirk (YF) or MITR. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.)
IP (Immunoprecipitation)
(This figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL).)
IP (Immunoprecipitation)
(This figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL).)
WB (Western Blot)
(Both anti-HDAC9 N-term (AAA284307) and C-term (AP1109b) Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both Pab specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone.)
WB (Western Blot)
(Both anti-HDAC9 N-term (AAA284307) and C-term (AP1109b) Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both Pab specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone.)
Related Product Information for anti-HDAC9 antibody
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.
Product Categories/Family for anti-HDAC9 antibody
References
Petrie, K., et al., J. Biol. Chem. 278(18):16059-16072 (2003). David, D., et al., Genomics 81(5):489-503 (2003). Mahlknecht, U., et al., Biochem. Biophys. Res. Commun. 293(1):182-191 (2002). Zhou, X., et al., Proc. Natl. Acad. Sci. U.S.A. 98(19):10572-10577 (2001). Zhang, C.L., et al., J. Biol. Chem. 276(1):35-39 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
111297
NCBI Official Full Name
histone deacetylase 9 isoform 6
NCBI Official Synonym Full Names
histone deacetylase 9
NCBI Official Symbol
HDAC9
NCBI Official Synonym Symbols
HD7; HD9; HD7b; HDAC; HDRP; MITR; HDAC7; HDAC7B; HDAC9B; HDAC9FL
NCBI Protein Information
histone deacetylase 9
UniProt Protein Name
Histone deacetylase 9
UniProt Gene Name
HDAC9
UniProt Synonym Gene Names
HDAC7; HDAC7B; HDRP; KIAA0744; MITR; HD9; HD7; HD7b
UniProt Entry Name
HDAC9_HUMAN
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Product Notes
The HDAC9 hdac9 (Catalog #AAA284307) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-32. The HDAC9 Antibody (N-term) reacts with Human (Predicted Reactivity: Chicken, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC9 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), IHC (Immunohistochemistry), ELISA, IP (Immunoprecipitation). Researchers should empirically determine the suitability of the HDAC9 hdac9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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