IP (Immunoprecipitation)
(Detection of human HDGF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HDGF antibody AAA212128 used for IP at 6 ug/mg lysate. HDGF was also immunoprecipitated by rabbit anti-HDGF antibodies and which recognize downstream epitopes. For blotting immunoprecipitated HDGF, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Rabbit anti-Human, Mouse HDGF Polyclonal Antibody | anti-HDGF antibody
Rabbit anti-HDGF Antibody, Affinity Purified
Gene Names
HDGF; HMG1L2
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
HDGF, Antibody; Rabbit anti-HDGF Antibody, Affinity Purified; high mobility group protein 1-like 2; epididymis secretory sperm binding protein; HMG1L2; HMG-1L2; HDGF; hepatoma derived growth factor; hepatoma-derived growth factor; Hepatoma-derived growth factor; heparin binding growth factor; anti-HDGF antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-HDGF antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Rat
Immunogen
Between 125 and 175
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human HDGF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HDGF antibody AAA212128 used for IP at 6 ug/mg lysate. HDGF was also immunoprecipitated by rabbit anti-HDGF antibodies and which recognize downstream epitopes. For blotting immunoprecipitated HDGF, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human HDGF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HDGF antibody AAA212128 used for IP at 6 ug/mg lysate. HDGF was also immunoprecipitated by rabbit anti-HDGF antibodies and which recognize downstream epitopes. For blotting immunoprecipitated HDGF, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohiostchemistry)
(Detection of mouse HDGF by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-HDGF (Cat. No. AAA212128 Lot1, left image, Lot1, middle image and Cat. No. Lot1, right image) used at a dilution of 1:1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of mouse HDGF by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-HDGF (Cat. No. AAA212128 Lot1, left image, Lot1, middle image and Cat. No. Lot1, right image) used at a dilution of 1:1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human HDGF by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-HDGF (Cat. No. AAA212128 Lot1, left image, Lot1, middle image and Cat. No. Lot1, right image) used at a dilution of 1:1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human HDGF by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-HDGF (Cat. No. AAA212128 Lot1, left image, Lot1, middle image and Cat. No. Lot1, right image) used at a dilution of 1:1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
Related Product Information for anti-HDGF antibody
Hepatoma-derived growth factor (HDGF) was identified as a mitogen secreted from the human hepatoma cell line Huh-7. HDGF has been found to bind DNA and to function as a transcriptional repressor.
Product Categories/Family for anti-HDGF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,788 Da
NCBI Official Full Name
hepatoma-derived growth factor isoform a
NCBI Official Synonym Full Names
hepatoma-derived growth factor
NCBI Official Symbol
HDGF
NCBI Official Synonym Symbols
HMG1L2
NCBI Protein Information
hepatoma-derived growth factor; HMG-1L2; high mobility group protein 1-like 2
UniProt Protein Name
Hepatoma-derived growth factor
UniProt Gene Name
HDGF
UniProt Synonym Gene Names
HMG1L2; HDGF; HMG-1L2
UniProt Entry Name
HDGF_HUMAN
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Product Notes
The HDGF hdgf (Catalog #AAA212128) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-HDGF Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HDGF can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the HDGF hdgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDGF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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