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WB (Western Blot)
(Western Blot; Sample: Lane1: 293T cell lysate; Lane2: A431 cell lysate; Lane3: A549 cell lysate; Lane4: Hela cell lysate; Lane5: K562 cell lysate Primary Ab: 0.2ug/ml Rabbit Anti-human HSP90b1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Rabbit anti-Human, Mouse Heat Shock Protein 90kDa Beta 1 (HSP90b1) Polyclonal Antibody | anti-HSP90b1 antibody
Polyclonal Antibody to Heat Shock Protein 90kDa Beta 1 (HSP90b1)
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Heat Shock Protein 90kDa Beta 1 (HSP90b1), Antibody; Polyclonal Antibody to Heat Shock Protein 90kDa Beta 1 (HSP90b1); Heat Shock Protein 90kDa Beta 1; HSP90-B1; ECGP; GP96; GRP94; TRA1; Glycoprotein 96; Tumor Rejection Antigen(gp96)1;94 kDa glucose-reulated protein; Endoplasmin; anti-HSP90b1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against HSP90b1. It has been selected for its ability to recognize HSP90b1 in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.23mg/mL (varies by lot)
Sequence Positions
Asp22~Asn502
Applicable Applications for anti-HSP90b1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-2ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL
Immunogen
Recombinant Heat Shock Protein 90kDa Beta 1 (HSP90b1)
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Western Blot; Sample: Lane1: 293T cell lysate; Lane2: A431 cell lysate; Lane3: A549 cell lysate; Lane4: Hela cell lysate; Lane5: K562 cell lysate Primary Ab: 0.2ug/ml Rabbit Anti-human HSP90b1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Lane1: 293T cell lysate; Lane2: A431 cell lysate; Lane3: A549 cell lysate; Lane4: Hela cell lysate; Lane5: K562 cell lysate Primary Ab: 0.2ug/ml Rabbit Anti-human HSP90b1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Mouse Liver TissuePrimary Ab: 20ug/ml Rabbit Anti-human HSP90b1 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Mouse Liver TissuePrimary Ab: 20ug/ml Rabbit Anti-human HSP90b1 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Sample: Mouse Testis TissuePrimary Ab: 20ug/ml Rabbit Anti-human HSP90b1 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Sample: Mouse Testis TissuePrimary Ab: 20ug/ml Rabbit Anti-human HSP90b1 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Mouse CerebuM TissuePrimary Ab: 20ug/ml Rabbit Anti-human HSP90b1 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Mouse CerebuM TissuePrimary Ab: 20ug/ml Rabbit Anti-human HSP90b1 AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Mouse Kidney Tissue; Primary Ab: 20ug/ml Rabbit Anti-human HSP90b1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Mouse Kidney Tissue; Primary Ab: 20ug/ml Rabbit Anti-human HSP90b1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Mouse Liver Tissue; Primary Ab: 20ug/ml Rabbit Anti-human HSP90b1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Mouse Liver Tissue; Primary Ab: 20ug/ml Rabbit Anti-human HSP90b1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Mouse Testis Tissue; Primary Ab: 20ug/ml Rabbit Anti-human HSP90b1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Mouse Testis Tissue; Primary Ab: 20ug/ml Rabbit Anti-human HSP90b1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Sample: Mouse CerebuM Tissue; Primary Ab: 20ug/ml Rabbit Anti-human HSP90b1 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Sample: Mouse CerebuM Tissue; Primary Ab: 20ug/ml Rabbit Anti-human HSP90b1 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Product Categories/Family for anti-HSP90b1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,469 Da
NCBI Official Full Name
endoplasmin
NCBI Official Synonym Full Names
heat shock protein 90kDa beta (Grp94), member 1
NCBI Official Symbol
HSP90B1
NCBI Official Synonym Symbols
ECGP; GP96; TRA1; GRP94
NCBI Protein Information
endoplasmin; tumor rejection antigen 1; 94 kDa glucose-regulated protein; tumor rejection antigen (gp96) 1; endothelial cell (HBMEC) glycoprotein; heat shock protein 90 kDa beta member 1; stress-inducible tumor rejection antigen gp96
UniProt Protein Name
Endoplasmin
UniProt Gene Name
HSP90B1
UniProt Synonym Gene Names
GRP94; TRA1; GRP-94
UniProt Entry Name
ENPL_HUMAN
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Product Notes
The HSP90b1 hsp90b1 (Catalog #AAA21253) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Asp22~Asn502. The Polyclonal Antibody to Heat Shock Protein 90kDa Beta 1 (HSP90b1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Heat Shock Protein 90kDa Beta 1 (HSP90b1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-2ug/mL IHC: 5-20ug/mL ICC: 5-20ug/mL. Researchers should empirically determine the suitability of the HSP90b1 hsp90b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock Protein 90kDa Beta 1 (HSP90b1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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