IHC (Immunohiostchemistry)
((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat GPR94/TRA1 Polyclonal Antibody | anti-HSP90B1 antibody
Goat anti-GPR94/TRA1 Antibody
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow, Zebrafish
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
GPR94/TRA1, Antibody; Goat anti-GPR94/TRA1 Antibody; TRA1; GRP94; tumor rejection antigen (gp96) 1; HGNC: 12028; ECGP; GP96; Tumor rejection antigen-1 (gp96); endothelial cell (HBMEC) glycoprotein; glucose regulated protein, 94 kDa; TRA1 antibody; GRP94 antibody; tumor rejection antigen (gp96) 1 antibody; HGNC: 12028 antibody; ECGP antibody; GP96 antibody; Tumor rejection antigen-1 (gp96) antibody; endothelial cell (HBMEC) glycoprotein antibody; glucose regulated protein; 94 kDa antibody; GPR94 / TRA1; anti-HSP90B1 antibody
Host
Goat
Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow, Zebrafish
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KEGVKFDESEKTKE
Sequence Length
803
Applicable Applications for anti-HSP90B1 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-KEGVKFDESEKTKE, from the internal region of the protein sequence according to NP_003290.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
IHC (Immunohiostchemistry)
((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohiostchemistry)
((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
WB (Western Blot)
((0. 03ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
((0. 03ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,469 Da
NCBI Official Full Name
endoplasmin
NCBI Official Synonym Full Names
heat shock protein 90kDa beta family member 1
NCBI Official Symbol
HSP90B1
NCBI Official Synonym Symbols
ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
NCBI Protein Information
endoplasmin
UniProt Protein Name
Endoplasmin
UniProt Gene Name
HSP90B1
UniProt Synonym Gene Names
GRP94; TRA1; GRP-94
UniProt Entry Name
ENPL_HUMAN
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Product Notes
The HSP90B1 hsp90b1 (Catalog #AAA60916) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GPR94/TRA1 Antibody reacts with Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's GPR94/TRA1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the HSP90B1 hsp90b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KEGVKFDESE KTKE. It is sometimes possible for the material contained within the vial of "GPR94/TRA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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