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WB (Western Blot)
(Applications: Western BlotSample: Whole cell extract of pancreatic tumor cell MIA PaACa2Detection: Enhanced chemiluminescence"Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (AAA46047) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected.")
Rabbit Heme oxygenase 1 Polyclonal Antibody | anti-HMOX1 antibody
Anti-HMOX1 Antibody
Gene Names
HMOX1; HO-1; HSP32; HMOX1D; bK286B10
Reactivity
Human, Rat. No cross reactivity with other proteins.
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Heme oxygenase 1, Antibody; Anti-HMOX1 Antibody; 32 kD antibody; bK286B10 antibody; D8Wsu38e antibody; heat shock protein 32 kD antibody; heat shock protein 32kD antibody; Heat shock protein antibody; Heme oxygenase (decycling) 1 antibody; Heme oxygenase 1 antibody; Hemox antibody; HMOX 1 antibody; Hmox antibody; HMOX1 antibody; HMOX1_HUMAN antibody; HO 1 antibody; HO antibody; HO-1 antibody; HO1 antibody; Hsp32 antibody; heme oxygenase (decycling) 1; anti-HMOX1 antibody
Host
Rabbit
Reactivity
Human, Rat. No cross reactivity with other proteins.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
288
Applicable Applications for anti-HMOX1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E.coli-derived human HMOX1 recombinant protein (Position: M1-M288). Human HMOX1 shares 82% and 80% amino acid (aa) sequences identity with mouse and rat HMOX1, respectively.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Applications: Western BlotSample: Whole cell extract of pancreatic tumor cell MIA PaACa2Detection: Enhanced chemiluminescence"Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (AAA46047) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected.")
WB (Western Blot)
(Applications: Western BlotSample: Whole cell extract of pancreatic tumor cell MIA PaACa2Detection: Enhanced chemiluminescence"Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (AAA46047) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected.")
IHC (Immunohistochemisry)
(Anti- HMOX1 antibody, AAA46047, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohistochemisry)
(Anti- HMOX1 antibody, AAA46047, IHC(P)IHC(P): Human Lung Cancer Tissue)
WB (Western Blot)
(Anti- HMOX1 antibody, AAA46047, Western blottingAll lanes: Anti HMOX1 (AAA46047) at 0.5ug/mlLane 1: Human Placenta Tissue Lysate at 50ugLane 2: Rat Spleen Tissue Lysate at 50ugLane 3: A549 Whole Cell Lysate at 40ugLane 4: PANC Whole Cell Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugPredicted bind size: 33KDObserved bind size: 33KD)
WB (Western Blot)
(Anti- HMOX1 antibody, AAA46047, Western blottingAll lanes: Anti HMOX1 (AAA46047) at 0.5ug/mlLane 1: Human Placenta Tissue Lysate at 50ugLane 2: Rat Spleen Tissue Lysate at 50ugLane 3: A549 Whole Cell Lysate at 40ugLane 4: PANC Whole Cell Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugPredicted bind size: 33KDObserved bind size: 33KD)
WB (Western Blot)
(Anti- HMOX1 antibody, AAA46047, Western blottingAll lanes: Anti HMOX1 (AAA46047) at 0.5ug/mlWB: Recombinant Human HMOX1 Protein 0.5ngPredicted bind size: 37KDObserved bind size: 37KD)
WB (Western Blot)
(Anti- HMOX1 antibody, AAA46047, Western blottingAll lanes: Anti HMOX1 (AAA46047) at 0.5ug/mlWB: Recombinant Human HMOX1 Protein 0.5ngPredicted bind size: 37KDObserved bind size: 37KD)
Related Product Information for anti-HMOX1 antibody
Description: Rabbit IgG polyclonal antibody for Heme oxygenase 1(HMOX1) detection. Tested with WB, IHC-P in Human;Rat.
Background: HMOX1 (heme oxygenase (decycling) 1), also known as HO-1, is a human gene that encodes for the enzyme heme oxygenase 1. It is an essential enzyme in heme catabolism, it cleaves heme to form biliverdin. HMOX1 belongs to the heme oxygenase family. The HMOX1 gene is located on the long (q) arm of chromosome 22 at position 12.3, from base pair 34,101,636 to base pair 34,114,748. HMOX1, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. HMOX1 activity is induced by its substrate heme and by various nonheme substances.
Background: HMOX1 (heme oxygenase (decycling) 1), also known as HO-1, is a human gene that encodes for the enzyme heme oxygenase 1. It is an essential enzyme in heme catabolism, it cleaves heme to form biliverdin. HMOX1 belongs to the heme oxygenase family. The HMOX1 gene is located on the long (q) arm of chromosome 22 at position 12.3, from base pair 34,101,636 to base pair 34,114,748. HMOX1, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. HMOX1 activity is induced by its substrate heme and by various nonheme substances.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,819 Da
NCBI Official Full Name
heme oxygenase 1
NCBI Official Synonym Full Names
heme oxygenase (decycling) 1
NCBI Official Symbol
HMOX1
NCBI Official Synonym Symbols
HO-1; HSP32; HMOX1D; bK286B10
NCBI Protein Information
heme oxygenase 1; heat shock protein, 32-kD
UniProt Protein Name
Heme oxygenase 1
UniProt Gene Name
HMOX1
UniProt Synonym Gene Names
HO; HO1; HO-1
UniProt Entry Name
HMOX1_HUMAN
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Product Notes
The HMOX1 hmox1 (Catalog #AAA46047) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HMOX1 Antibody reacts with Human, Rat. No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Heme oxygenase 1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HMOX1 hmox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heme oxygenase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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