<
IHC (Immunohistochemistry)
(AAA329119 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit Hemoglobin Polyclonal Antibody | anti-HBB antibody
Hemoglobin subunit beta Antibody
Gene Names
HBB; ECYT6; CD113t-C; beta-globin
Reactivity
Human, Mouse, MonkeyPredicted Reactivity: Pig(100%), Bovine(86%), Horse(86%), Rabbit(100%), Chicken(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Hemoglobin, Antibody; Hemoglobin subunit beta Antibody; ba1; Beta 1 globin; beta globin; beta globin chain; BETA GLOBIN TYPE; BETA THALASSEMIAS; Beta-A1-globin; CD113t C; CD113t-C; ERYTHREMIA; HBB; Hemoglobin beta 1 chain; hemoglobin beta chain; hemoglobin beta chain complex; Hemoglobin beta chain; major form; HEMOGLOBIN BETA LOCUS; Hemoglobin beta-1 chain; Hemoglobin subunit beta-1; METHEMOGLOBINEMIA; anti-HBB antibody
Host
Rabbit
Reactivity
Human, Mouse, Monkey
Predicted Reactivity: Pig(100%), Bovine(86%), Horse(86%), Rabbit(100%), Chicken(88%)
Predicted Reactivity: Pig(100%), Bovine(86%), Horse(86%), Rabbit(100%), Chicken(88%)
Clonality
Polyclonal
Isotype
IgG
Specificity
Hemoglobin subunit beta Antibody detects endogenous levels of total Hemoglobin subunit beta.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
147
Applicable Applications for anti-HBB antibody
ELISA
Immunogen
A synthesized peptide derived from human Hemoglobin subunit beta, corresponding to a region within N-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(AAA329119 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(AAA329119 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from COS-7, using Hemoglobin Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from COS-7, using Hemoglobin Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from K562, using Hemoglobin Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from K562, using Hemoglobin Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using Hemoglobin Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using Hemoglobin Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-HBB antibody
Function: Involved in oxygen transport from the lung to the various peripheral tissues.LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure.functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation.
Post Translational Modifications: Glucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus.S-nitrosylated; a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2).Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure.
Tissue Specificity: Red blood cells.
Subunit Structure: Heterotetramer of two alpha chains and two beta chains in adult hemoglobin A (HbA). Heterotetramer of two zeta chains and two beta chains in hemoglobin Portland-2, detected in fetuses and neonates with homozygous alpha-thalassemia.
Similarity: Belongs to the globin family.
Post Translational Modifications: Glucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus.S-nitrosylated; a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2).Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure.
Tissue Specificity: Red blood cells.
Subunit Structure: Heterotetramer of two alpha chains and two beta chains in adult hemoglobin A (HbA). Heterotetramer of two zeta chains and two beta chains in hemoglobin Portland-2, detected in fetuses and neonates with homozygous alpha-thalassemia.
Similarity: Belongs to the globin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)15kD.
Predicted Molecular Weight: (Calculated)16kDa.
Predicted Molecular Weight: (Calculated)16kDa.
NCBI Official Full Name
Hemoglobin subunit beta
NCBI Official Synonym Full Names
hemoglobin subunit beta
NCBI Official Symbol
HBB
NCBI Official Synonym Symbols
ECYT6; CD113t-C; beta-globin
NCBI Protein Information
hemoglobin subunit beta
UniProt Protein Name
Hemoglobin subunit beta
UniProt Gene Name
HBB
UniProt Entry Name
HBB_HUMAN
Similar Products
Product Notes
The HBB hbb (Catalog #AAA329119) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hemoglobin subunit beta Antibody reacts with Human, Mouse, Monkey Predicted Reactivity: Pig(100%), Bovine(86%), Horse(86%), Rabbit(100%), Chicken(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's Hemoglobin can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the HBB hbb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hemoglobin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.