WB (Western Blot)
(Western blot analysis of extracts of mouse brain tissue, using HEPACAM antibody.)
Rabbit HEPACAM Polyclonal Antibody | anti-HEPACAM antibody
HEPACAM Antibody
Gene Names
HEPACAM; HEPN1; MLC2A; MLC2B; GlialCAM
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
HEPACAM, Antibody; HEPACAM Antibody; FLJ25530; GlialCAM; HECAM_HUMAN; HEPACAM; Hepatocyte cell adhesion molecule; Protein hepaCAM; anti-HEPACAM antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
HEPACAM antibody detects endogenous levels of HEPACAM.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
416
Applicable Applications for anti-HEPACAM antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human HEPACAM.
Subcellular Location
Cytoplasm. Membrane. In MCF7 breast carcinoma and hepatic Hep3B and HepG2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1.
Tissue Specificity
Down-regulated in 20 out of 23 of hepatocellular carcinoma (HCC) samples and is undetectable in 5 HCC cell lines tested.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Zebrafish (80%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of mouse brain tissue, using HEPACAM antibody.)
WB (Western Blot)
(Western blot analysis of extracts of mouse brain tissue, using HEPACAM antibody.)
IHC (Immunohistochemistry)
(AAA322902 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA322902 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-HEPACAM antibody
Function: Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation.
Subunit Structure: Homodimer. Dimer formation occurs predominantly through cis interactions on the cell surface. Part of a complex containing MLC1, TRPV4, AQP4 and ATP1B1.
Post-translational Modifications: N-glycosylated.
Similarity: The cytoplasmic domain plays an important role in regulation of cell-matrix adhesion and cell motility.
Subunit Structure: Homodimer. Dimer formation occurs predominantly through cis interactions on the cell surface. Part of a complex containing MLC1, TRPV4, AQP4 and ATP1B1.
Post-translational Modifications: N-glycosylated.
Similarity: The cytoplasmic domain plays an important role in regulation of cell-matrix adhesion and cell motility.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 46-72 kDa
Predicted: 47 kDa
Predicted: 47 kDa
NCBI Official Full Name
hepatocyte cell adhesion molecule
NCBI Official Synonym Full Names
hepatic and glial cell adhesion molecule
NCBI Official Symbol
HEPACAM
NCBI Official Synonym Symbols
HEPN1; MLC2A; MLC2B; GlialCAM
NCBI Protein Information
hepatocyte cell adhesion molecule
UniProt Protein Name
Hepatocyte cell adhesion molecule
UniProt Gene Name
HEPACAM
UniProt Synonym Gene Names
Protein hepaCAM
Similar Products
Product Notes
The HEPACAM hepacam (Catalog #AAA322902) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HEPACAM Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HEPACAM can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HEPACAM hepacam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HEPACAM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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