IHC (Immunohiostchemistry)
(Anti-HEXA antibody, AAA45070, IHC(P)IHC(P):Human Intestinal Cancer Tissue)
Rabbit HEXA Polyclonal Antibody | anti-HEXA antibody
Anti-HEXA antibody
Gene Names
HEXA; TSD
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
HEXA, Antibody; Anti-HEXA antibody; Beta-hexosaminidase subunit alpha; hexosaminidase A(alpha polypeptide); Beta hexosaminidase alpha chain precursor antibody; Beta hexosaminidase subunit alpha antibody; Beta N acetylhexosaminidase antibody; Beta N acetylhexosaminidase subunit alpha antibody; Beta-hexosaminidase subunit alpha antibody; Beta-N-acetylhexosaminidase subunit alpha antibody; Hexa antibody; HEXA_HUMAN antibody; Hexosaminidase A(alpha polypeptide) antibody; Hexosaminidase A alpha polypeptide antibody; Hexosaminidase A antibody; Hexosaminidase subunit A antibody; MGC99608 antibody; N acetyl beta glucosaminidase antibody; N acetyl beta glucosaminidase subunit alpha antibody; N-acetyl-beta-glucosaminidase subunit alpha antibody; TSD antibody; anti-HEXA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
529
Applicable Applications for anti-HEXA antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human HEXA (191-207aa DVMAYNKLNVFHWHLVD), different from the related rat and mouse sequences by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti-HEXA antibody, AAA45070, IHC(P)IHC(P):Human Intestinal Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti-HEXA antibody, AAA45070, IHC(P)IHC(P):Human Intestinal Cancer Tissue)
WB (Western Blot)
(Anti-HEXA antibody, AAA45070, Western blottingAll lanes: Anti HEXA (AAA45070) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugPredicted bind size: 61KDObserved bind size: 61KD)
WB (Western Blot)
(Anti-HEXA antibody, AAA45070, Western blottingAll lanes: Anti HEXA (AAA45070) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugPredicted bind size: 61KDObserved bind size: 61KD)
Related Product Information for anti-HEXA antibody
Description: Rabbit IgG polyclonal antibody for Beta-hexosaminidase subunit alpha(HEXA) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: HEXA(hexosaminidase A(alpha polypeptide)) is an enzyme that in humans is encoded by the HEXA gene. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines The HEXA gene encodes the alpha subunit of hexosaminidase A, a lysosomal enzyme involved in the breakdown of gangliosides. The HEXA gene is mapped on 15q23. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit is able to hydrolyze GM2 gangliosides. The alpha subunit contains a key residue, Arg-424, which is essential for binding the N-acetyl-neuramanic residue of GM2 gangliosides. Chimeric constructs were expressed in HeLa cells and selected constructs were produced in the baculovirus expression system to determine their ability to degrade GM2 ganglioside in the presence of GM2 activator protein. Their results allowed them to define 2 noncontiguous sequences in the alpha subunit(amino acids 1-191 and 403-529) which, when substituted into analogous positions in the beta subunit, conferred activity against the sulfated substrate.
Background: HEXA(hexosaminidase A(alpha polypeptide)) is an enzyme that in humans is encoded by the HEXA gene. Hexosaminidase A and the cofactor GM2 activator protein catalyze the degradation of the GM2 gangliosides and other molecules containing terminal N-acetyl hexosamines The HEXA gene encodes the alpha subunit of hexosaminidase A, a lysosomal enzyme involved in the breakdown of gangliosides. The HEXA gene is mapped on 15q23. Even though the alpha and beta subunits of hexosaminidase A can both cleave GalNAc residues, only the alpha subunit is able to hydrolyze GM2 gangliosides. The alpha subunit contains a key residue, Arg-424, which is essential for binding the N-acetyl-neuramanic residue of GM2 gangliosides. Chimeric constructs were expressed in HeLa cells and selected constructs were produced in the baculovirus expression system to determine their ability to degrade GM2 ganglioside in the presence of GM2 activator protein. Their results allowed them to define 2 noncontiguous sequences in the alpha subunit(amino acids 1-191 and 403-529) which, when substituted into analogous positions in the beta subunit, conferred activity against the sulfated substrate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
19,326 Da
NCBI Official Full Name
Beta-hexosaminidase subunit alpha
NCBI Official Synonym Full Names
hexosaminidase A (alpha polypeptide)
NCBI Official Symbol
HEXA
NCBI Official Synonym Symbols
TSD
NCBI Protein Information
beta-hexosaminidase subunit alpha; hexosaminidase subunit A; beta-N-acetylhexosaminidase subunit alpha; N-acetyl-beta-glucosaminidase subunit alpha
UniProt Protein Name
Beta-hexosaminidase subunit alpha
UniProt Gene Name
HEXA
UniProt Synonym Gene Names
Hexosaminidase subunit A
UniProt Entry Name
HEXA_HUMAN
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Product Notes
The HEXA hexa (Catalog #AAA45070) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HEXA antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HEXA can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HEXA hexa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HEXA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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