WB (Western Blot)
(Detection of human HEXIM1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HEXIM1 antibody AAA212113 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies and which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).)
Rabbit anti-Human, Mouse HEXIM1 Polyclonal Antibody | anti-HEXIM1 antibody
Rabbit anti-HEXIM1 Antibody, Affinity Purified
Gene Names
HEXIM1; CLP1; EDG1; HIS1; MAQ1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
HEXIM1, Antibody; Rabbit anti-HEXIM1 Antibody, Affinity Purified; cardiac lineage protein 1; hexamthylene bis-acetamide inducible 1; protein HEXIM1; menage a quatre protein 1; menage a quatre 1; MAQ1; HMBA-inducible; HIS1; hexamethylene-bis-acetamide-inducible transcript 1; hexamethylene bis-acetamide-inducible protein 1; hexamethylene bisacetamide-inducible protein; hexamethylene bis-acetamide inducible 1; estrogen down-regulated gene 1 protein; CLP1; EDG1; Protein HEXIM1; hexamethylene bisacetamide inducible 1; anti-HEXIM1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-HEXIM1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Bovine
Immunogen
Between 200 and 250
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human HEXIM1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HEXIM1 antibody AAA212113 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies and which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human HEXIM1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HEXIM1 antibody AAA212113 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies and which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).)
IHC (Immunohiostchemistry)
(Detection of mouse HEXIM1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-HEXIM1 (Cat. No. AAA212113 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of mouse HEXIM1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-HEXIM1 (Cat. No. AAA212113 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human HEXIM1 by immunohistochemistry. Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti-HEXIM1 (Cat. No. Lot1, left image, AAA212113 Lot1, middle image and Cat. No. Lot1, right image) used at a dilution of 1:200 (1ug/ml) or 1:1,000 (1ug/ml) (AAA212113-1 and Detection: DAB. Counterstain: hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human HEXIM1 by immunohistochemistry. Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti-HEXIM1 (Cat. No. Lot1, left image, AAA212113 Lot1, middle image and Cat. No. Lot1, right image) used at a dilution of 1:200 (1ug/ml) or 1:1,000 (1ug/ml) (AAA212113-1 and Detection: DAB. Counterstain: hematoxylin (blue).)
Related Product Information for anti-HEXIM1 antibody
Hexamethylene bis-acetamide-inducible protein 1 (HEXIM1) is a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA, HEXIM1sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. HEXIM1 may also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O94992].
Product Categories/Family for anti-HEXIM1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,623 Da
NCBI Official Full Name
protein HEXIM1
NCBI Official Synonym Full Names
hexamethylene bis-acetamide inducible 1
NCBI Official Symbol
HEXIM1
NCBI Official Synonym Symbols
CLP1; EDG1; HIS1; MAQ1
NCBI Protein Information
protein HEXIM1; HMBA-inducible; menage a quatre 1; cardiac lineage protein 1; menage a quatre protein 1; estrogen down-regulated gene 1 protein; hexamthylene bis-acetamide inducible 1; hexamethylene bisacetamide-inducible protein; hexamethylene bis-acetam
UniProt Protein Name
Protein HEXIM1
UniProt Gene Name
HEXIM1
UniProt Synonym Gene Names
CLP1; EDG1; HIS1; MAQ1
UniProt Entry Name
HEXI1_HUMAN
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Product Notes
The HEXIM1 hexim1 (Catalog #AAA212113) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-HEXIM1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HEXIM1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the HEXIM1 hexim1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HEXIM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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