Application Data
(AAA329285 staining Hela cells(heat shock treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1%Triton X-100,then blocked in 10% serum for 45 minutes at25°C. Samples were then incubated with primary Ab(AAA329285)and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red)and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
Rabbit anti-Human, Rat Hexokinase 1 Polyclonal Antibody | anti-HK1 antibody
Phospho-Hexokinase 1 (Tyr732) Antibody
Gene Names
HK1; HKI; HXK1; HK1-ta; HK1-tb; HK1-tc
Reactivity
Human, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
Peptide affinity purification
Synonyms
Hexokinase 1, Antibody; Phospho-Hexokinase 1 (Tyr732) Antibody; BB404130; Brain form hexokinase; dea; DrHXK1; EC 2.7.1.1; Glycolytic enzyme; HEXOKIN; hexokinase I; Hexokinase PI; Hexokinase type I; Hexokinase, tumor isozyme; Hexokinase-1; Hexokinase-A; HK I; HK1; HK1 tb; Hk1-s; HK1-ta; HK1-tb; HK1-tc; HKD; HKI; HMSNR; HXK1; HXK1_HUMAN; im:7148527; mHk1-s; wu:fc09d08; wu:fc16e02; wu:fc21e02; wu:fq14b11; zgc:55790; zgc:77618; anti-HK1 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Specificity
Phospho-Hexokinase 1 (Tyr732) Antibody detects endogenous levels of Hexokinase 1 only when phosphorylated at Tyr732.
Purity/Purification
Peptide affinity purification
Form/Format
IgG in phosphate buffered saline , pH 7.4, 150mMNaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-HK1 antibody
ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry)
Immunogen
A synthesized peptide derived from human Hexokinase 1around the phosphorylation site of Tyr732.
Preparation and Storage
Store at -20°C.
Application Data
(AAA329285 staining Hela cells(heat shock treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1%Triton X-100,then blocked in 10% serum for 45 minutes at25°C. Samples were then incubated with primary Ab(AAA329285)and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red)and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
Application Data
(AAA329285 staining Hela cells(heat shock treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1%Triton X-100,then blocked in 10% serum for 45 minutes at25°C. Samples were then incubated with primary Ab(AAA329285)and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red)and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Human pancreatic cancer and adjacent nomal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Human pancreatic cancer and adjacent nomal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from PC12(lps4h treatment), using Phospho-Hexokinase 1 (Tyr732) Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from PC12(lps4h treatment), using Phospho-Hexokinase 1 (Tyr732) Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-HK1 antibody
Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
hexokinase-1 isoform HKI
NCBI Official Synonym Full Names
hexokinase 1
NCBI Official Symbol
HK1
NCBI Official Synonym Symbols
HKI; HXK1; HK1-ta; HK1-tb; HK1-tc
NCBI Protein Information
hexokinase-1; HK I; glycolytic enzyme; hexokinase type I; brain form hexokinase
UniProt Protein Name
Hexokinase-1
UniProt Gene Name
HK1
UniProt Synonym Gene Names
HK I
UniProt Entry Name
HXK1_HUMAN
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Product Notes
The HK1 hk1 (Catalog #AAA329285) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Hexokinase 1 (Tyr732) Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Hexokinase 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the HK1 hk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hexokinase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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