IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with HEYL Antibody (N-term). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.)
Rabbit HEYL Polyclonal Antibody | anti-HEYL antibody
HEYL Antibody (N-term)
Gene Names
HEYL; HEY3; HRT3; HESR3; bHLHb33
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
HEYL, Antibody; HEYL Antibody (N-term); Hairy/enhancer-of-split related with YRPW motif-like protein; hHeyL; Class B basic helix-loop-helix protein 33; bHLHb33; Hairy-related transcription factor 3; HRT-3; hHRT3; HEYL; BHLHB33; HRT3; anti-HEYL antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This HEYL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human HEYL.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
26-54
Sequence Length
328
Applicable Applications for anti-HEYL antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Function
Downstream effector of Notch signaling which may be required for cardiovascular development (By similarity). Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
Cellular Location
Nucleus {ECO:0000255|PROSITE-ProRule:PRU00380, ECO:0000255|PROSITE-ProRule:PRU00981}
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with HEYL Antibody (N-term). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with HEYL Antibody (N-term). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.)
WB (Western Blot)
(HEYL Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HEYL antibody detected the HEYL protein (arrow).)
WB (Western Blot)
(HEYL Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HEYL antibody detected the HEYL protein (arrow).)
Related Product Information for anti-HEYL antibody
This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq].
References
Kokubo, H., et al. Trends Cardiovasc. Med. 15(5):190-194(2005)
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
Iso, T., et al. J. Cell. Physiol. 194(3):237-255(2003)
Nakagawa, O., et al. Proc. Natl. Acad. Sci. U.S.A. 97(25):13655-13660(2000)
Firulli, B.A., et al. J. Biol. Chem. 275(43):33567-33573(2000)
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
Iso, T., et al. J. Cell. Physiol. 194(3):237-255(2003)
Nakagawa, O., et al. Proc. Natl. Acad. Sci. U.S.A. 97(25):13655-13660(2000)
Firulli, B.A., et al. J. Biol. Chem. 275(43):33567-33573(2000)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35087
NCBI Official Full Name
hairy/enhancer-of-split related with YRPW motif-like protein
NCBI Official Synonym Full Names
hes-related family bHLH transcription factor with YRPW motif-like
NCBI Official Symbol
HEYL
NCBI Official Synonym Symbols
HEY3; HRT3; HESR3; bHLHb33
NCBI Protein Information
hairy/enhancer-of-split related with YRPW motif-like protein
UniProt Protein Name
Hairy/enhancer-of-split related with YRPW motif-like protein
UniProt Gene Name
HEYL
UniProt Synonym Gene Names
BHLHB33; HRT3; hHeyL; bHLHb33; HRT-3; hHRT3
UniProt Entry Name
HEYL_HUMAN
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Product Notes
The HEYL heyl (Catalog #AAA287219) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 26-54. The HEYL Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's HEYL can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HEYL heyl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HEYL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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